Artículo
Kinetics of antigen cross-presentation assessed experimentally and by a model of the complete endomembrane system
Nieto Grimalt, Franco Emiliano
; Garrido, Facundo Matias
; Dinamarca, Sofía
; Cebrián, José Ignacio
; Mayorga, Luis Segundo
Fecha de publicación:
12/2022
Editorial:
Academic Press Inc Elsevier Science
Revista:
Cellular Immunology
ISSN:
0008-8749
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Dendritic cells (DCs) have a specialized endomembrane system capable of presenting exogenous antigens in the context of MHC class I (MHC-I) molecules. This process, named cross-presentation, is crucial to activate CD8+ T lymphocytes and initiate cytotoxic immune responses. In this report, we present an Agent-Based Model in combination with Ordinary Differential Equations with enough complexity to reproduce cross-presentation. The model embraces the secretory and endocytic pathways, in connection with the plasma membrane, the endoplasmic reticulum, and the cytosol. Key molecules required for cross-presentation were included as cargoes. In the simulations, the kinetics of MHC-I uptake and recycling, and cross-presentation accurately reproduced experimental values. The model proved to be a suitable tool to elaborate hypotheses and design experiments. In particular, the model predictions and the experimental results obtained indicate that the rate-limiting step in cross-presentation of soluble ovalbumin is MHC-I loading after proteasomal processing of the antigenic protein.
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Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Nieto Grimalt, Franco Emiliano; Garrido, Facundo Matias; Dinamarca, Sofía; Cebrián, José Ignacio; Mayorga, Luis Segundo; Kinetics of antigen cross-presentation assessed experimentally and by a model of the complete endomembrane system; Academic Press Inc Elsevier Science; Cellular Immunology; 382; 12-2022; 1-11
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