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dc.contributor.author
Silva, Carolina R.
dc.contributor.author
Lifschitz, Adrian Luis
dc.contributor.author
Macedo, Sara R. D.
dc.contributor.author
Campos, Nagilla R. C. L.
dc.contributor.author
Viana-Filho, Malaquias
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Alcântara, Ana C. S.
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Araújo, Josiel G.
dc.contributor.author
Alencar, Luciana M. R.
dc.contributor.author
Costa-Junior, Livio M.
dc.date.available
2023-07-13T20:13:21Z
dc.date.issued
2021-02
dc.identifier.citation
Silva, Carolina R.; Lifschitz, Adrian Luis; Macedo, Sara R. D.; Campos, Nagilla R. C. L.; Viana-Filho, Malaquias; et al.; Combination of synthetic anthelmintics and monoterpenes: Assessment of efficacy, and ultrastructural and biophysical properties of Haemonchus contortus using atomic force microscopy; Elsevier Science; Veterinary Parasitology; 290; 2-2021; 1-9
dc.identifier.issn
0304-4017
dc.identifier.uri
http://hdl.handle.net/11336/203877
dc.description.abstract
The resistance of Haemonchus contortus to synthetic anthelmintics is of increasing concern; and different strategies are being evaluated to improve parasite control. The present study investigated the in vitro effects of combinations of synthetic compounds and monoterpenes. Additionally, the chemical association of the best combinations and their impact on the ultrastructural and biophysical properties of H. contortus eggs was evaluated. We assessed the efficacy of the monoterpenes, carvacrol, thymol, r-carvone, s-carvone, citral, and p-cymene and the anthelmintics, albendazole and levamisole using the egg hatch test (EHT) and the larval migration inhibition test (LMIT), respectively. The minimum effective concentrations of the monoterpenes, according to the EHT (efficacy ranging from 4.4%–11.8%) and LMIT (efficacy ranging from 5.6%–7.4%), were used in combination with different concentrations of synthetic compounds, and the IC50 and synergism rate (SR) were calculated. Fourier-transform infrared spectroscopy (FTIR) was used to analyze the chemical association between the best combinations as revealed by the in vitro tests (albendazole and levamisole with r-carvone or s-carvone). Atomic force microscopy (AFM) was used to assess the ultrastructural and biophysical properties of H. contortus eggs treated with the albendazole and r-carvone combination. Among the monoterpenes, the highest efficacies were exhibited by carvacrol (IC50 = 185.9 μg/mL) and thymol (IC50 = 187.0 μg/mL), according to the EHT, and s-carvone and carvacrol (IC50 = 1526.0 and 1785.3 μg/mL, respectively), according to the LMIT. According to the EHT, albendazole showed a slight statistically significant synergism in combination with r-carvone (SR = 3.8) and s-carvone (SR = 3.0). According to the LMIT, among the monoterpenes, r-carvone (SR = 1.7) and s-carvone (SR = 1.7) showed an increase in efficacy with levamisole; however, this was not statistically significant. The FTIR spectra of albendazole and levamisole, in association with r-carvone and s-carvone, indicated the presence of chemical interactions between the synthetic and natural molecules, contributing to the possible synergistic effects of these associations. Eggs treated with albendazole and r-carvone showed an increase in roughness and a decrease in height, suggesting that the treatment induced damage to the egg surface and an overflow of its internal contents. Overall, the combination of albendazole with r-carvone and s-carvone was efficacious against H. contortus, demonstrating a chemical association between the compounds; the significant changes in the egg ultrastructure justify this efficacy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ALBENDAZOLE
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ATOMIC FORCE MICROSCOPY
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CARVONE
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IN VITRO
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LEVAMISOLE
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MONOTERPENES
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Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Combination of synthetic anthelmintics and monoterpenes: Assessment of efficacy, and ultrastructural and biophysical properties of Haemonchus contortus using atomic force microscopy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-06-28T15:26:51Z
dc.journal.volume
290
dc.journal.pagination
1-9
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Silva, Carolina R.. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Macedo, Sara R. D.. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Campos, Nagilla R. C. L.. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Viana-Filho, Malaquias. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Alcântara, Ana C. S.. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Araújo, Josiel G.. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Alencar, Luciana M. R.. Universidade Federal do Maranhao; Brasil
dc.description.fil
Fil: Costa-Junior, Livio M.. Universidade Federal do Maranhao; Brasil
dc.journal.title
Veterinary Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401721000054
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2021.109345
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