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dc.contributor.author
Aguinaga Martinez, Maite Victoria
dc.contributor.author
Jozičová, Natali
dc.contributor.author
Dušek, Jan
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Horstkotte, Burkhard
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Pávek, Petr
dc.contributor.author
Miró, Manuel
dc.contributor.author
Sklenářová, Hana
dc.date.available
2023-07-13T14:45:34Z
dc.date.issued
2022-08
dc.identifier.citation
Aguinaga Martinez, Maite Victoria; Jozičová, Natali; Dušek, Jan; Horstkotte, Burkhard; Pávek, Petr; et al.; Real-time monitoring of Metridia luciferase release from cells upon interaction with model toxic substances by a fully automatic flow setup – A proof of concept; Elsevier Science; Talanta; 245; 1; 8-2022; 1-7
dc.identifier.issn
0039-9140
dc.identifier.uri
http://hdl.handle.net/11336/203796
dc.description.abstract
This manuscript reports on a fully automatic sequential injection system incorporating a 3D printed module for real-time monitoring of the release of Metridia luciferase from a modified liver epithelial cell line. To this end, a simple and effective approach for the automation of flash-type chemiluminescence assays was developed. The 3D printed module comprised an apical and a basal compartment that enabled monitoring membrane processes on both sides of the cell monolayer aimed at elucidating the direction of luciferase release. A natural release was observed after transfection with the luciferase plasmid by online measurement of the elicited light from the reaction of the synthesized luciferase with the coelenterazine substrate. Model substances for acute toxicity from the group of cholic acids – chenodeoxycholic and deoxycholic acids – were applied at the 1.0 and 0.5 mmol L−1 levels. The tested cholic acids caused changes in cell membrane permeability that was accompanied by an increased luciferase release. The obtained kinetic profiles were evaluated based on the delay between the addition of the toxic substance and the increase of the chemiluminescence signal. All experiments were carried out in a fully automatic system in ca. 5 min per sample in 30 min intervals and no manual interventions were needed for a sampling period of at least 6 h.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
3D-PRINTING
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AUTOMATION
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CELL TOXICITY
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METRIDIA LUCIFERASE
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REAL-TIME MONITORING
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SECUENCIAL INJECTION ANALYSIS
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Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Real-time monitoring of Metridia luciferase release from cells upon interaction with model toxic substances by a fully automatic flow setup – A proof of concept
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-06T21:50:32Z
dc.journal.volume
245
dc.journal.number
1
dc.journal.pagination
1-7
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Aguinaga Martinez, Maite Victoria. Karlova Univerzita (cuni); . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
dc.description.fil
Fil: Jozičová, Natali. Karlova Univerzita (cuni);
dc.description.fil
Fil: Dušek, Jan. Karlova Univerzita (cuni);
dc.description.fil
Fil: Horstkotte, Burkhard. Karlova Univerzita (cuni);
dc.description.fil
Fil: Pávek, Petr. Karlova Univerzita (cuni);
dc.description.fil
Fil: Miró, Manuel. Karlova Univerzita (cuni); . Universidad de las Islas Baleares; España
dc.description.fil
Fil: Sklenářová, Hana. Karlova Univerzita (cuni);
dc.journal.title
Talanta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039914022002612
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.talanta.2022.123465
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