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dc.contributor.author
Adamczyk, Aleksandra K.
dc.contributor.author
Huijben, Teun A. P. M.
dc.contributor.author
Sison, Miguel
dc.contributor.author
Di Luca, Andrea
dc.contributor.author
Chiarelli, Germán
dc.contributor.author
Vanni, Stefano
dc.contributor.author
Brasselet, Sophie
dc.contributor.author
Mortensen, Kim I.
dc.contributor.author
Stefani, Fernando Daniel
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dc.contributor.author
Pilo-Pais, Mauricio
dc.contributor.author
Acuna, Guillermo P.
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dc.date.available
2023-07-13T14:31:14Z
dc.date.issued
2022-10
dc.identifier.citation
Adamczyk, Aleksandra K.; Huijben, Teun A. P. M.; Sison, Miguel; Di Luca, Andrea; Chiarelli, Germán; et al.; DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation; American Chemical Society; ACS Nano; 16; 10; 10-2022; 16924-16931
dc.identifier.issn
1936-0851
dc.identifier.uri
http://hdl.handle.net/11336/203780
dc.description.abstract
An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control, and well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up nanofabrication methodology that fulfils almost all of these features. It enables the nanometric positioning of molecules and nanoparticles with stoichiometric control, and even the orientation of asymmetrical nanoparticles along predefined directions. However, orienting individual molecules has been a standing challenge. Here, we show how single molecules, namely, Cy5 and Cy3 fluorophores, can be incorporated in a DNA origami with controlled orientation by doubly linking them to oligonucleotide strands that are hybridized while leaving unpaired bases in the scaffold. Increasing the number of bases unpaired induces a stretching of the fluorophore linkers, reducing its mobility freedom, and leaves more space for the fluorophore to accommodate and find different sites for interaction with the DNA. Particularly, we explore the effects of leaving 0, 2, 4, 6, and 8 bases unpaired and find extreme orientations for 0 and 8 unpaired bases, corresponding to the molecules being perpendicular and parallel to the DNA double-helix, respectively. We foresee that these results will expand the application field of DNA origami toward the fabrication of nanodevices involving a wide range of orientation-dependent molecular interactions, such as energy transfer, intermolecular electron transport, catalysis, exciton delocalization, or the electromagnetic coupling of a molecule to specific resonant nanoantenna modes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DNA NANOTECHNOLOGY
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DNA ORIGAMI
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NANOFABRICATION
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NANOPHOTONICS
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SINGLE-MOLECULE FLUORESCENCE
dc.subject.classification
Nano-materiales
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dc.subject.classification
Nanotecnología
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-10T11:52:11Z
dc.journal.volume
16
dc.journal.number
10
dc.journal.pagination
16924-16931
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Adamczyk, Aleksandra K.. Universite de Fribourg;
dc.description.fil
Fil: Huijben, Teun A. P. M.. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Sison, Miguel. Institut Fresnel; Francia
dc.description.fil
Fil: Di Luca, Andrea. Universite de Fribourg;
dc.description.fil
Fil: Chiarelli, Germán. Universite de Fribourg;
dc.description.fil
Fil: Vanni, Stefano. Universite de Fribourg;
dc.description.fil
Fil: Brasselet, Sophie. Institut Fresnel; Francia
dc.description.fil
Fil: Mortensen, Kim I.. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
dc.description.fil
Fil: Pilo-Pais, Mauricio. Universite de Fribourg;
dc.description.fil
Fil: Acuna, Guillermo P.. Universite de Fribourg;
dc.journal.title
ACS Nano
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acsnano.2c06936
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