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dc.contributor.author
Ribeiro, Isabelle de Paula
dc.contributor.author
Guerra Pinto, Juliana
dc.contributor.author
Müller Nunes Souza, Beatriz
dc.contributor.author
Miñan, Alejandro Guillermo
dc.contributor.author
Ferreira Strixino, Juliana
dc.date.available
2023-07-11T12:59:18Z
dc.date.issued
2022-03
dc.identifier.citation
Ribeiro, Isabelle de Paula; Guerra Pinto, Juliana; Müller Nunes Souza, Beatriz; Miñan, Alejandro Guillermo; Ferreira Strixino, Juliana; Antimicrobial photodynamic therapy with curcumin on methicillin-resistant Staphylococcus aureus biofilm; Elsevier Science; Photodiagnosis Photodynamic Therapy; 37; 3-2022; 1-8
dc.identifier.issn
1572-1000
dc.identifier.uri
http://hdl.handle.net/11336/203188
dc.description.abstract
Healthcare-Associated Infections (HAI) effect approximately 1.5 million individuals worldwide. Among the causes of HAIs in Latin America, Staphylococcus aureus presents a severe danger due to its rapid spread and ease of developing antibiotic resistance. Upon acquiring methicillin resistance, it receives the classification Methicillin-Resistant Staphylococcus aureus (MRSA), responsible for 40 to 60% of HAIs. The increase in resistant microorganisms led to the search for alternative methods, such as antimicrobial Photodynamic Therapy (aPDT), forming Reactive Oxygen Species (ROS), leading bacterial cells to death. The objective of this work was to evaluate in vitro the antimicrobial action of PDT with curcumin in MRSA biofilm. The strains were induced to form biofilm and incubated with curcumin for 20 min, irradiated with LED (Light Emitting Diode) 450 nm, at 110 mW/cm2, 50 J/cm2 for 455 s, subsequently counting the Colony Forming Units, Scanning Electron Microscopy (SEM) micrographs, Confocal Microscopy images, Resazurin dye test, ROS quantification to assess the effect of PDT on biofilm. The results show that PDT with curcumin reduced the biofilm growth of the MRSA strain. In addition, confocal microscopy showed that curcumin was internalized by S. aureus in the cells at the concentration used, and when isolated, curcumin and the irradiation parameter did not show cytotoxicity. The study demonstrated that the PDT in the established parameters reduced the growth of the MRSA strain biofilm, making it a relevant alternative possibility for the inactivation of this strain.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIMICROBIAL PHOTODYNAMIC THERAPY
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BIOFILM
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CURCUMIN
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S. AUREUS
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Antimicrobial photodynamic therapy with curcumin on methicillin-resistant Staphylococcus aureus biofilm
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-05T12:20:47Z
dc.journal.volume
37
dc.journal.pagination
1-8
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Ribeiro, Isabelle de Paula. Universidade Estadual da Paraiba; Brasil
dc.description.fil
Fil: Guerra Pinto, Juliana. Universidade Estadual da Paraiba; Brasil
dc.description.fil
Fil: Müller Nunes Souza, Beatriz. Universidade Estadual da Paraiba; Brasil
dc.description.fil
Fil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
dc.description.fil
Fil: Ferreira Strixino, Juliana. Universidade Estadual da Paraiba; Brasil
dc.journal.title
Photodiagnosis Photodynamic Therapy
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.pdpdt.2022.102729
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