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dc.contributor.author
Chapela, Sebastián P.
dc.contributor.author
Burgos, Hilda Isabel
dc.contributor.author
Stella, Carlos Alberto
dc.date.available
2023-07-10T18:05:26Z
dc.date.issued
2022-02-05
dc.identifier.citation
Chapela, Sebastián P.; Burgos, Hilda Isabel; Stella, Carlos Alberto; N-Acetyl cysteine improves cellular growth in respiratory-deficient yeast; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 53; 2; 5-2-2022; 791-794
dc.identifier.issn
1517-8382
dc.identifier.uri
http://hdl.handle.net/11336/203050
dc.description.abstract
Background: Reactive oxygen species (ROS) is a main factor that alters cellular physiology and functionality. Many strategies are used in order to control excessive oxidative stress. One strategy includes the use of antioxidants like N-acetyl cysteine (NAC). The aim of this study was to compare the effect of this antioxidant on ROS production and cellular growth of a wild-type and a respiratory-deficient Saccharomyces cerevisiae strain. Methods: Using a simple system such as yeast allows oxidative stress investigations on which numerous factors are more manageable or circumscribed than in a higher organism. We grew cells in a complex medium and incubated them during 72 h. Later, cellular viability and ROS production was evaluated. ROS level was estimated by use of fluorescence signal with 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Results: As it is found in the present work, a reducing environment exerted by NAC presence during incubation of the cells allows a respiratory-deficient Saccharomyces cerevisiae strain to improve its cellular growth. Conclusions: It seems likely that the energy production or the phenotype which characterizes a deficient strain is incapable of palliating ROS growth inhibition while NAC helps to overcome this limitation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedade Brasileira de Microbiologia
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIOXIDANT EFFECT
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REACTIVE OXYGEN SPECIES (ROS)
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YEAST
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Métodos de Investigación en Bioquímica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
N-Acetyl cysteine improves cellular growth in respiratory-deficient yeast
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-07T22:57:20Z
dc.identifier.eissn
1678-4405
dc.journal.volume
53
dc.journal.number
2
dc.journal.pagination
791-794
dc.journal.pais
Brasil
dc.journal.ciudad
Sao Paulo
dc.description.fil
Fil: Chapela, Sebastián P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina. Hospital Británico de Buenos Aires; Argentina
dc.description.fil
Fil: Burgos, Hilda Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Stella, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.journal.title
Brazilian Journal of Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s42770-022-00705-5
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