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Artículo

Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant

Camussone, Cecilia MaríaIcon ; Pujato, NazarenaIcon ; Renna, Maria SolIcon ; Veaute, Carolina Melania Isabel; Morein, B.; Marcipar, Ivan SergioIcon ; Calvinho, Luis Fernando
Fecha de publicación: 12/2014
Editorial: Elsevier
Revista: Veterinary Immunology And Immunopathology
ISSN: 0165-2427
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnología Agropecuaria

Resumen

Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 lysate vaccine adjuvanted with Iscom Matrix to generate a longer lasting specific antibody response in blood and milk, with improved opsonic capacity, compared with a S. aureus CP5 whole-cell formulation. The aim of the present study was to obtain an experimental immunogen composed of lysed cells of a CP5 S. aureus strain supplemented with recombinant clumping factor A, fibronectin binding protein A and -toxin formulated with Iscom Matrix, characterize the immune response generated when immunizing pregnant heifers and assess the functional role of antibodies raised against this immunogen in experimental models. Both a lysate vaccine and a lysate + recombinant antigens vaccine elicited antibodies that promoted neutrophil phagocytosis and inhibited internalization into mammary epithelial cells, in vitro. Incorporation of defined antigenic molecules to the lysate formulation elicited a strong specific humoral immune response against both lysate and recombinant antigens and was associated with higher expression of regulatory and pro-inflammatory cytokines. In addition, antibodies were efficient for blocking S. aureus binding to bovine fibrinogen and fibronectin, and neutralizing -toxin effect in vitro, placing these antigens as candidates to be included in a formulation directed to prevent staphylococcal bovine mastitis.
Palabras clave: Staphylococcus Aureus , Bovine Mastitis , Immunization , Lysate , Recombinant Antigens , Iscom Matrix
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/20296
DOI: http://dx.doi.org/10.1016/j.vetimm.2014.10.004
URL: http://www.sciencedirect.com/science/article/pii/S0165242714002293
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Articulos(ICIVET-LITORAL)
Articulos de INST. DE CIENCIAS VETERINARIAS DEL LITORAL
Citación
Camussone, Cecilia María; Pujato, Nazarena; Renna, Maria Sol; Veaute, Carolina Melania Isabel; Morein, B.; et al.; Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant; Elsevier; Veterinary Immunology And Immunopathology; 162; 3-4; 12-2014; 96-107
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