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Artículo

Recovery of acid proteases from fishery discards with aqueous micellar two-phase systems and their use for X-ray film recycling

Acevedo Gomez, Antonella ValeriaIcon ; Bustillo, Soledad; Nerli, Bibiana BeatrizIcon
Fecha de publicación: 11/2022
Editorial: Elsevier
Revista: Process Biochemistry
ISSN: 1359-5113
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Ingeniería de Procesos Químicos

Resumen

The continuous expansion of fish production results in increasing amounts of discards, i.e. viscera, skin and frame, whose inappropriate disposal causes environmental damage. Conversion of this waste into value-added products is one of the best alternatives to reduce the pollution problem. The feasibility of using aqueous micellar two-phase systems (AMTPS), formed by biodegradable Genapol X-080 (GX-080) and sodium citrate, to recover valued acid proteases from surubí (Pseudoplatystoma sp) fishery discards was evaluated for the first time. The effects of biomass load, pH and surfactant concentration on the extraction were analyzed using a two-level full factorial design. GX-080 concentration and biomass load were the most significant factors (p < 0.05). AMTPS formed by GX-080 4% w/w showed a successful performance for the recovering of 69% of AP from the crude extract in the surfactant-rich phase with a purification factor of 1.83. Regard to the storage stability, this extract conserved at least 89% of its proteolytic activity for 76 days at 0 °C. It also showed a notable hydrolyzing activity of the gelatin-silver coating of used X-ray films, thus opening new perspectives on the use of acid proteases for recycling purposes.
Palabras clave: ACID PROTEASES , AQUEOUS MICELLAR TWO-PHASE SYSTEMS , CLOUD POINT EXTRACTION , FISHERY BY-PRODUCTS , SURUBÍ (PSEUDOPLATYSTOMA SP) , X-RAY FILM RECYCLING
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/202904
URL: https://linkinghub.elsevier.com/retrieve/pii/S1359511322003737
DOI: https://dx.doi.org/10.1016/j.procbio.2022.10.016
Colecciones
Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos(IQUIBA-NEA)
Articulos de INSTITUTO DE QUIMICA BASICA Y APLICADA DEL NORDESTE ARGENTINO
Citación
Acevedo Gomez, Antonella Valeria; Bustillo, Soledad; Nerli, Bibiana Beatriz; Recovery of acid proteases from fishery discards with aqueous micellar two-phase systems and their use for X-ray film recycling; Elsevier; Process Biochemistry; 122; 11-2022; 194-204
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