Show simple item record

dc.contributor.author de La Vega Beltrán, José Luis
dc.contributor.author Sánchez Cárdenas, Claudia
dc.contributor.author Krapf, Dario
dc.contributor.author Hernández González, Enrique
dc.contributor.author Wertheimer Hermitte, Eva Victoria
dc.contributor.author Trevinio, Claudia L.
dc.contributor.author Visconti, Pablo E.
dc.contributor.author Darszon, Alberto
dc.date.available 2017-07-12T20:51:33Z
dc.date.issued 2012-12
dc.identifier.citation de La Vega Beltrán, José Luis; Sánchez Cárdenas, Claudia; Krapf, Dario; Hernández González, Enrique; Wertheimer Hermitte, Eva Victoria; et al.; Mouse sperm membrane potential hyperpolarization is necessary and sufficient to prepare sperm for the acrosome reaction; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 287; 53; 12-2012; 44384-44393
dc.identifier.issn 0021-9258
dc.identifier.uri http://hdl.handle.net/11336/20286
dc.description.abstract Mammalian sperm are unable to fertilize the egg immediately after ejaculation; they acquire this capacity during migration in the female reproductive tract. This maturational process is called capacitation and in mouse sperm it involves a plasma membrane reorganization, extensive changes in the state of protein phosphorylation, increases in intracellular pH (pHi) and Ca2+ ([Ca2+]i), and the appearance of hyperactivated motility. In addition, mouse sperm capacitation is associated with the hyperpolarization of the cell membrane potential. However, the functional role of this process is not known. In this work, to dissect the role of this membrane potential change, hyperpolarization was induced in noncapacitated sperm using either the ENaC inhibitor amiloride, the CFTR agonist genistein or the K+ ionophore valinomycin. In this experimental setting, other capacitation-associated processes such as activation of a cAMP-dependent pathway and the consequent increase in protein tyrosine phosphorylation were not observed. However, hyperpolarization was sufficient to prepare sperm for the acrosome reaction induced either by depolarization with high K+ or by addition of solubilized zona pellucida (sZP). Moreover, K+ and sZP were also able to increase [Ca2+]i in non-capacitated sperm treated with these hyperpolarizing agents but not in untreated cells. On the other hand, in conditions that support capacitation-associated processes blocking hyperpolarization by adding valinomycin and increasing K+ concentrations inhibited the agonist-induced acrosome reaction as well as the increase in [Ca2+]i. Altogether, these results suggest that sperm hyperpolarization by itself is key to enabling mice sperm to undergo the acrosome reaction.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society for Biochemistry and Molecular Biology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CALCIUM IMAGING
dc.subject FERTILIZATION
dc.subject ION CHANNELS
dc.subject PHOSPHORYLATION
dc.subject SPERM
dc.subject ACROSOME REACTION
dc.subject CALCIUM
dc.subject HYPERPOLARIZATION
dc.subject SPERM CAPACITATION
dc.subject.classification Biología Reproductiva
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification Fisiología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Mouse sperm membrane potential hyperpolarization is necessary and sufficient to prepare sperm for the acrosome reaction
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-12T13:19:02Z
dc.journal.volume 287
dc.journal.number 53
dc.journal.pagination 44384-44393
dc.journal.pais Estados Unidos
dc.journal.ciudad Bethesda
dc.description.fil Fil: de La Vega Beltrán, José Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil Fil: Hernández González, Enrique. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados; México
dc.description.fil Fil: Wertheimer Hermitte, Eva Victoria. University of Massachussets; Estados Unidos
dc.description.fil Fil: Trevinio, Claudia L.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
dc.description.fil Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.journal.title Journal of Biological Chemistry
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/53/44384
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M112.393488
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531752/


Archivos asociados

This item appears in the following Collection(s)

  • Articulos(IBR) [320]
    Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
  • Articulos(CEFYBO) [328]
    Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS

Show simple item record

info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)