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dc.contributor.author
Turani, Ornella  
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Hernando, Guillermina Silvana  
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Bouzat, Cecilia Beatriz  
dc.date.available
2023-06-30T11:55:36Z  
dc.date.issued
2021  
dc.identifier.citation
Unravelling the physiological role and molecular function of betaine-sensitive nicotinic receptors of Caenorhabditis elegans; XXXVI Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; Reunión Virtual; Argentina; 2021; 226-226  
dc.identifier.uri
http://hdl.handle.net/11336/201891  
dc.description.abstract
The free-living nematode Caenorhabditis elegans is a model of parasitic nematodes. It has one of the most extensive nicotinic receptor (nAChR) families. However, the molecular functional properties and roles of many of these nAChRs remained unknown. ACR-23 is a nAChR present in neuronal and muscle cells of nematodes and it is not conserved in vertebrates. It is a cation-selective channel activated by betaine (BE) and sensitive to monepantel (MNP), a novel anthelmintic drug. Given the limited information about its functional role in nematodes, we explored ACR-23 from a physiological and molecular perspective. Locomotion assays of adult worm showed that BE significantly increased their motility. This effect was not observed in acr-23 mutants, indicating that BE acts through ACR-23. Interestingly, BE did not affect L1, indicating differential sensitivity between stages, probably arising from changes in ACR-23 expression levels during development. MNP decreased worm motility in the adult stage in a concentration-dependent manner with an EC50 of ~30 µM. The acr-23 mutant showed different MNP sensitivity compared to the wild-type strain, indicating that besides ACR-23 other receptors may be targeted by MNP. By using a primary culture of C. elegans muscle cells, we described for the first time the properties of BE-elicited single-channel currents. Our study provides insights into the molecular basis of anthelmintic action, which pave the way for the development of novel drugs.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedad Argentina de Investigación en Neurociencias  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
NEMATODES  
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NICOTINIC RECEPTORS  
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BETAINE  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Unravelling the physiological role and molecular function of betaine-sensitive nicotinic receptors of Caenorhabditis elegans  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2023-06-01T10:09:26Z  
dc.journal.pagination
226-226  
dc.journal.pais
Argentina  
dc.journal.ciudad
Buenos Aires  
dc.description.fil
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.description.fil
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.description.fil
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/  
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Autor  
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Autor  
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Autor  
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Nacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
XXXVI Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias  
dc.date.evento
2021-10-18  
dc.description.ciudadEvento
Reunión Virtual  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación en Neurociencias  
dc.source.libro
SAN2021 XXXVI Annual Meeting  
dc.date.eventoHasta
2021-10-22  
dc.type
Reunión