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Ferragut, Fatima Eneida del Valle  
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Alcaráz, Paula B.  
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Chadi, Raul  
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Fernandez, Marisa  
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Hernandez Vasquez, Yolanda Maria  
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Gomez, Karina Andrea  
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Acevedo, Gonzalo Raúl  
dc.date.available
2023-06-29T16:23:36Z  
dc.date.issued
2021  
dc.identifier.citation
Expression and functionality of co-inhibitory receptors TIGIT, TIM-3 and LAG-3 in CD4+ T cells from patients with chronic Chagas disease; XXXVI Meeting of the Brazilian Society of Protozoology; XLVII Annual Meeting on Basic Research in Chagas’ Disease; Sao Paulo; Brasil; 2021; 34-34  
dc.identifier.uri
http://hdl.handle.net/11336/201804  
dc.description.abstract
Chagas disease, caused by Trypanosoma cruzi, affects 7 million people worldwide, mainly in Latin America where it is endemic. Unless treated early after infection, the disease progresses to a chronic form in which some patients develop cardiac or digestive alterations, while others stay asymptomatic. CD4+ T cell response plays critical and diverse roles during infection, and becomes impaired over time with defective cytokine release. This process known as T cell exhaustion is also defined by the upregulation of inhibitory receptors, mainly PD-1 and CTLA-4. In this work, we aimed to explore the expression of the second line of inhibitory receptors TIGIT, TIM-3 and LAG-3 in parasite stimulated CD4 T cells from patients with different stages of chronic Chagas disease (CCD), and whether their blockade restores cell functionality. Antigen-specific CD4 T cells were identified by activation induced markers (AIM) assay, using the surface molecules Ox40 and CD25. CCD patients, independently of their clinical stage, showed an increased frequency of CD4+TIGIT+ T cells. TIM-3+ CD4+ T cells were more abundant in patients with cardiac manifestations, while LAG-3+ cells were increased in asymptomatic CCD patients, with higher frequency within the non-activated cells subpopulation upon T. cruzi lysate stimulation. Preliminary data showed that TIM-3 blockade tended to increase IFN-g+ CD4+ T cells in CCD patients with cardiomyopathy. Furthermore, in the same group of subjects, the incubation with anti-TIGIT blocking antibody led to a greater frequency of IL-10+ CD4+ T cells compared to the isotype control antibody. Our results highlight the role of the inhibitory receptors TIGIT, TIM-3 and LAG-3 in the modulation of anti-T. cruzi CD4+ T cell responses, in relation with the progression of chronic Chagas disease, and paves the way for the development of novel therapeutic strategies.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedade Brasileira de Protozoologia  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
TRYPANOSOMA CRUZI  
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INHIBITORY RECEPTORS  
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T CELLS  
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CHAGAS DISEASE  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Expression and functionality of co-inhibitory receptors TIGIT, TIM-3 and LAG-3 in CD4+ T cells from patients with chronic Chagas disease  
dc.type
info:eu-repo/semantics/publishedVersion  
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info:eu-repo/semantics/conferenceObject  
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info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-11-09T18:27:18Z  
dc.journal.pagination
34-34  
dc.journal.pais
Brasil  
dc.journal.ciudad
Sao Paulo  
dc.description.fil
Fil: Ferragut, Fatima Eneida del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
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Fil: Alcaráz, Paula B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
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Fil: Chadi, Raul. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina  
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Fil: Fernandez, Marisa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina  
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Fil: Hernandez Vasquez, Yolanda Maria. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina  
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Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
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Fil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://sbpz.org.br/wp-content/uploads/2021/08/Livro-resumos-2021.pdf  
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Internacional  
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Reunión  
dc.description.nombreEvento
XXXVI Meeting of the Brazilian Society of Protozoology; XLVII Annual Meeting on Basic Research in Chagas’ Disease  
dc.date.evento
2021-08-30  
dc.description.ciudadEvento
Sao Paulo  
dc.description.paisEvento
Brasil  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedade Brasileira de Protozoologia  
dc.source.libro
Proceedings XXXVI Meeting of the Brazilian Society of Protozoology - XLVII Annual Meeting on Basic Research in Chagas’ Disease  
dc.date.eventoHasta
2021-09-01  
dc.type
Reunión