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dc.contributor.author
Chowdhury, Jamil
dc.contributor.author
Kemppainen, Minna Johanna
dc.contributor.author
Delhomme, Nicolas
dc.contributor.author
Shutava, Iryna
dc.contributor.author
Zhou, Jingjing
dc.contributor.author
Takahashi, Junko
dc.contributor.author
Pardo, Alejandro Guillermo
dc.contributor.author
Lundberg-Felten, Judith
dc.date.available
2023-06-28T15:43:34Z
dc.date.issued
2022-10
dc.identifier.citation
Chowdhury, Jamil; Kemppainen, Minna Johanna; Delhomme, Nicolas; Shutava, Iryna; Zhou, Jingjing; et al.; Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides; Wiley Blackwell Publishing, Inc; New Phytologist; 236; 2; 10-2022; 639-655
dc.identifier.issn
0028-646X
dc.identifier.uri
http://hdl.handle.net/11336/201690
dc.description.abstract
The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de-esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM-induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de-esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
CELL WALL
dc.subject
CELL-WALL REMODELLING
dc.subject
CELL-WALL-MODIFYING ENZYMES
dc.subject
ECTOMYCORRHIZA
dc.subject
LACCARIA BICOLOR
dc.subject
PECTIN
dc.subject
PECTIN METHYLESTERASE
dc.subject
POPULUS
dc.subject.classification
Micología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-06-26T16:17:49Z
dc.journal.volume
236
dc.journal.number
2
dc.journal.pagination
639-655
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Chowdhury, Jamil. Universidad de Umea; Suecia
dc.description.fil
Fil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
dc.description.fil
Fil: Delhomme, Nicolas. Universidad de Umea; Suecia
dc.description.fil
Fil: Shutava, Iryna. Universidad de Umea; Suecia
dc.description.fil
Fil: Zhou, Jingjing. Universidad de Umea; Suecia
dc.description.fil
Fil: Takahashi, Junko. Universidad de Umea; Suecia
dc.description.fil
Fil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
dc.description.fil
Fil: Lundberg-Felten, Judith. Universidad de Umea; Suecia
dc.journal.title
New Phytologist
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18358
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/nph.18358
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