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dc.contributor.author
Peñalva, Daniel Alejandro
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dc.contributor.author
Monnappa, Ajay K.
dc.contributor.author
Natale, Paolo
dc.contributor.author
López Montero, Iván
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dc.date.available
2023-06-28T13:36:24Z
dc.date.issued
2022
dc.identifier.citation
Fusion of micron-size vesicles: interplay between the mitochondrial Mfn2 protein and lipids; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Argentina; 2022; 84-84
dc.identifier.uri
http://hdl.handle.net/11336/201610
dc.description.abstract
Membrane fusion is crucial for the coordination of mitochondrial dynamics. an imbalanced mitochondrial dynamic leads to the formation of fragmented mitochondria and a decrease in intracellular atp levels, contributing to the development of important diseases, including neurodegenerative, cardiac or cancer conditions. the fusion process is energetically unfavorable, thereby requiring specialized proteins. in mammals, mitofusins (mfn) 1 and 2 are responsible for mitochondrial outer-membrane (omm) fusion. they belong to the dynamin superfamily of multi-domain gtpases. recent structural studies suggest that, upon gtp hydrolysis, mfns dimerize to promote the approaching and fusion of omm. however, the omm fusion seems to require multiple regulatory factors that control the dynamics and kinetics of mitochondrial fusion throught the formation of heterotypic mfn1- mfn2 dimers. in this study, we purified and functionally reconstituted the full-length mouse mfn2 in large and giant unilamellar vesicles (luvs and guvs, respectively). vesicles were prepared with popc alone or with 30% of plasmalogen-pc or dope. unlike gdp, after incubation with gtp, vesicles underwent fusion. fast video microscopy imaged the mfn2-dependent membrane fusion pathway which involves the formation and expansion of a membrane diaphragm and the opening of a fusion pore. the incorporation of dope (30% mol) in the lipid composition did not alter the fusion sequence but enhanced the fusion kinetics significantly, as revealed by a lipid-mixing assay. our observations show that mfn2 alone can promote the fusion of micron-sized vesicles, without the presence of other proteins in the membrane. in addition, the lipid environment is an important factor in the modulation of mfn2-dependent membrane fusion, a process that seems to require topological lipid intermediates with negative curvature
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedad Argentina de Investigaciones en Bioquimica y Biología Molecular
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MITOFUSIN
dc.subject
MEMBRANE FUSION
dc.subject
MITOCHONDRIA
dc.subject
LIPID VESICLES
dc.subject.classification
Biofísica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Fusion of micron-size vesicles: interplay between the mitochondrial Mfn2 protein and lipids
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-06-15T18:02:43Z
dc.journal.pagination
84-84
dc.journal.pais
Argentina
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dc.journal.ciudad
Mendoza
dc.description.fil
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Monnappa, Ajay K.. Hospital Doce de Octubre; España
dc.description.fil
Fil: Natale, Paolo. Hospital Doce de Octubre; España. Universidad Complutense de Madrid; España
dc.description.fil
Fil: López Montero, Iván. Universidad Complutense de Madrid; España
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/congreso2022/
dc.conicet.rol
Autor
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Autor
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Autor
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dc.conicet.rol
Autor
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dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
dc.date.evento
2022-11-08
dc.description.paisEvento
Argentina
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dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
dc.source.libro
Congreso SAIB 2022: Resúmenes de comunicaciones orales y posters
dc.source.revista
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
dc.date.eventoHasta
2022-11-11
dc.type
Congreso
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