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dc.contributor.author
Chequer Charan, Daniela Maria
dc.contributor.author
Hua, Yunfeng
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Wang, Haoyu
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Huang, Wenqing
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Wang, Fangfang
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Elgoyhen, Ana Belen
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Boergens, Kevin M.
dc.contributor.author
Di Guilmi, Mariano Nicolás
dc.date.available
2023-06-26T11:22:38Z
dc.date.issued
2022-12-15
dc.identifier.citation
Chequer Charan, Daniela Maria; Hua, Yunfeng; Wang, Haoyu; Huang, Wenqing; Wang, Fangfang; et al.; Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem; Frontiers Media; Frontiers in Cellular Neuroscience; 16; 15-12-2022; 1-16
dc.identifier.issn
1662-5102
dc.identifier.uri
http://hdl.handle.net/11336/201416
dc.description.abstract
The medial nucleus of the trapezoid body (MNTB) is an integral component of the auditory brainstem circuitry involved in sound localization. The giant presynaptic nerve terminal with multiple active zones, the calyx of Held (CH), is a hallmark of this nucleus, which mediates fast and synchronized glutamatergic synaptic transmission. To delineate how these synaptic structures adapt to reduced auditory afferents due to aging, we acquired and reconstructed circuitry-level volumes of mouse MNTB at different ages (3 weeks, 6, 18, and 24 months) using serial block-face electron microscopy. We used C57BL/6J, the most widely inbred mouse strain used for transgenic lines, which displays a type of age-related hearing loss. We found that MNTB neurons reduce in density with age. Surprisingly we observed an average of approximately 10% of poly-innervated MNTB neurons along the mouse lifespan, with prevalence in the low frequency region. Moreover, a tonotopy-dependent heterogeneity in CH morphology was observed in young but not in older mice. In conclusion, our data support the notion that age-related hearing impairments can be in part a direct consequence of several structural alterations and circuit remodeling in the brainstem.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
MNTB
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Aging
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Serial block electron microscopy
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-06-22T10:19:28Z
dc.identifier.eissn
1662-5102
dc.journal.volume
16
dc.journal.pagination
1-16
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
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Fil: Hua, Yunfeng. Shanghai Institute Of Precision Medicine; China
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Fil: Wang, Haoyu. Shanghai Institute Of Precision Medicine; China
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Fil: Huang, Wenqing. Shanghai Institute Of Precision Medicine; China
dc.description.fil
Fil: Wang, Fangfang. Shanghai Institute Of Precision Medicine; China
dc.description.fil
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Boergens, Kevin M.. University of Illinois; Estados Unidos
dc.description.fil
Fil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.journal.title
Frontiers in Cellular Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2022.1070438/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fncel.2022.1070438
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