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dc.contributor.author
Aparicio, Juan Daniel  
dc.contributor.author
Urionabarrenetxea, Erik  
dc.contributor.author
García Velasco, Nerea  
dc.contributor.author
Soto López, Manuel  
dc.date.available
2023-06-23T20:27:42Z  
dc.date.issued
2017  
dc.identifier.citation
Assessment of the toxic effects exerted by chromium in soils combining standardized OECD tests and cell-based approaches in earthworms (Eisenia fetida); 27th Annual Meeting of the Society of Environmental Toxicology and Chemistry Europe; Bruselas; Bélgica; 2017; 228-229  
dc.identifier.issn
2309-8031  
dc.identifier.uri
http://hdl.handle.net/11336/201385  
dc.description.abstract
Chromium (Cr) is widely used in a variety of industrial processes which lead to releases into the different environmental compartments. Inputs of Cr into terrestrial environments could modify soil community and affect soil health. The standardized earthworm Acute Toxicity Test (ATT) (OECD-207) with Eisenia fetida provides information about which concentrations of Cr pose effects on growth and mortality in earthworms. Complementarily, parameters measured in earthworms immune cells (coelomocytes), could be an accurate tool to assess the toxic effects exerted by Cr in these soil dwelling organisms. OECD soils (OECD-207, 1984) were contaminated with 10 - 150 mg Cr/kg (Cr was added as K2Cr2O7) and left stabilizing for 3 weeks. Healthy and clitellated E. fetida earthworms of similar size (400 ± 100 mg fresh weigh) were weight in tens and introduced in the test soils, maintained at 19 °C, in constant humidity and light conditions. For the ATT mortality and weight loss were measured (14 d) in order to obtain LC50 and EC50 values.. Cr was quantified in soils (EPA 3051A method), lixiviates (soluble fraction, DIN 38414-4) and earthworms (n=15) by AAS (SGIker General Service; UPV/EHU). Coelomocytes were extruded from exposed earthworms (3 pools of 5 individuals per treatment) and their number and cell viability were calculated. Cr concentrations in lixiviates were very low and suggest the formation of Cr-soil complexes while Cr was accumulated in earthworms showing an increasing gradient from 40 mg Cr/kg onwards. Mortality and weight loss followed a dose response fashion. Exposure to the highest doses (130 - 150 mg Cr/kg) produced 100 % mortality. The LC50 value was calculated as 97 mg Cr/kg, which corresponded to 20 mg Cr/kg soluble fraction. The EC50 was 115 mg Cr/kg and corresponded to 22 mg Cr/kg (soluble fraction). Regression analyses showed a significant reduction of cell number with exposure concentrations (R2 = 0.97, p<0.05). Coelomocytes decrease could be a first sign of more severe toxicological effects at higher levels of biological complexity. The NR uptake capacity of coelomocytes appeared to be affected at concentrations higher than 40 mg Cr/kg. Effects reported in the present work could be related to responses at lower levels of biological complexity such as oxidative stress, DNA damage and apoptosis. Acknowledgements - Funded by Basque Government (Consolidated Research Groups, IT810-13) and BEC.AR (Program of scholarships for Argentine professionals).  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Society of Environmental Toxicology and Chemistry  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
EISENIA FETIDA  
dc.subject
CR(VI)  
dc.subject
OECD TESTS  
dc.subject
ECOTOXICOLOGY  
dc.subject.classification
Biotecnología Medioambiental  
dc.subject.classification
Biotecnología del Medio Ambiente  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Assessment of the toxic effects exerted by chromium in soils combining standardized OECD tests and cell-based approaches in earthworms (Eisenia fetida)  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2023-06-21T15:46:32Z  
dc.identifier.eissn
2310-3043  
dc.journal.pagination
228-229  
dc.journal.pais
Bélgica  
dc.journal.ciudad
Bruselas  
dc.description.fil
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Urionabarrenetxea, Erik. Universidad del País Vasco; España  
dc.description.fil
Fil: García Velasco, Nerea. Universidad del País Vasco; España  
dc.description.fil
Fil: Soto López, Manuel. Universidad del País Vasco; España  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.setac.org/store/ViewProduct.aspx?ID=9195405  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Internacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
27th Annual Meeting of the Society of Environmental Toxicology and Chemistry Europe  
dc.date.evento
2017-05-07  
dc.description.ciudadEvento
Bruselas  
dc.description.paisEvento
Bélgica  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Society of Environmental Toxicology and Chemistry  
dc.source.libro
SETAC Europe 27th Annual Meeting Abstract Book  
dc.date.eventoHasta
2017-05-11  
dc.type
Reunión