Artículo
A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
Gonzalez Viacava, Myriam Belen; Leiva, Carlos Leónidas
; Tavarone, Maria Eugenia
; Dokmetjian, Jose Christian; Cascone, Osvaldo
; Chacana, Pablo Anibal
; Fingermann, Matias
Fecha de publicación:
11/2022
Editorial:
Elsevier Science
Revista:
Journal of Chromatography B
ISSN:
1570-0232
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms.
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Articulos(NANOBIOTEC)
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Gonzalez Viacava, Myriam Belen; Leiva, Carlos Leónidas; Tavarone, Maria Eugenia; Dokmetjian, Jose Christian; Cascone, Osvaldo; et al.; A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction; Elsevier Science; Journal of Chromatography B; 1211; 11-2022; 1-9
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