Evento
Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells
Tipo del evento:
Workshop
Nombre del evento:
Eleventh Multidisciplinary Workshop
Fecha del evento:
12/2009
Institución Organizadora:
Sociedad Argentina de Biología;
Título de la revista:
Biocell
Editorial:
Tech Science Press
ISSN:
0327-9545
Idioma:
Inglés
Clasificación temática:
Resumen
In previous works we described changes in the orexinergic hypo thalamus-pituitary-ovarian system in adult rats (AJP-EM 292: E820- E828, 2007; AJP-EM, 293: E977-85. 2007). Here we continue to study in vitro in ovaries of superovulated prepubertal rats (SPO) the presence of OX1 and OX2 receptors and the effects of orexin A (OXA) and B (OXB) on their expression. SPO rat ovarian cells were cultured and stimulated for 48 h with OXA (10-9M) and OXB (10-9M) in the presence or absence of their specific antagonists. We evaluated the secretion of progesterone released into the me dium by RIA. The quantification of the receptors was determined by real-time RT-PCR as in AJP-EM 293: E977-85. 2007. [...]
Palabras clave:
OREXIN
,
OVARY
,
RECEPTOR
,
CULTIVO
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Eventos(IBYME)
Eventos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Eventos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells; Eleventh Multidisciplinary Workshop; Buenos Aires; Argentina; 2009; 91-91
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