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dc.contributor.author
Puente, M. A.
dc.contributor.author
Tartaglione, C. M.
dc.contributor.author
Ritta, Monica Nora
dc.date.available
2023-06-08T12:09:54Z
dc.date.issued
2011-08
dc.identifier.citation
Puente, M. A.; Tartaglione, C. M.; Ritta, Monica Nora; Bull sperm acrosome reaction induced by gamma-aminobutyric acid (GABA) is mediated by GABAergic receptors type A; Elsevier Science; Animal Reproduction Science; 127; 1-2; 8-2011; 31-37
dc.identifier.issn
0378-4320
dc.identifier.uri
http://hdl.handle.net/11336/199961
dc.description.abstract
The effect of gamma-aminobutyric acid (GABA) on the bull sperm acrosome reaction was evaluated, and the interaction of progesterone, a physiologic inducer of the acrosome reaction, with the GABA receptor was explored. The acrosome reaction was stimulated by GABA in a dose-dependent manner. This effect was inhibited completely by bicuculline, a GABA A receptor antagonist, but GABA B and C receptor antagonists had no effect. Accordingly, muscimol, a GABA A receptor agonist, stimulated the acrosome reaction to the same extent as GABA, whereas baclofen (GABA B receptor agonist) and CACA (GABA C receptor agonist), had no effect. Preincubation with progesterone followed by the addition of GABA resulted in a significantincrease in the percentage of acrosome reacted spermatozoa compared with progesterone or GABA alone. Taking into account that this increase was less than a simple addition of effects, it might be suggested that GABA and progesterone act through the same receptor and/or use the same mechanism of action. To test this hypothesis, the abilities of GABA and progesterone to induce acrosome reaction were tested in the presence of bicuculline, which suppressed both stimulatory effects. Given thatthe GABA A receptor is linked to the Cl− channel, we tested whether picrotoxin, a blocker of this channel, could modulate the effects of progesterone or GABA. Cl− channel blocker picrotoxin dramatically reduced the GABA and progesterone-initiated AR. In conclusion: GABA and progesterone stimulate the acrosome reaction in bull spermatozoa acting through a classical GABA A receptor. The mechanism of action requires the functional integrity of the Ca2+ Cl− channel.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACROSOME REACTION
dc.subject
BULL SPERM
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CALCIUM CHANNEL
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CL- CHANNEL
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GABA
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GABA A RECEPTOR
dc.subject.classification
Otras Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Bull sperm acrosome reaction induced by gamma-aminobutyric acid (GABA) is mediated by GABAergic receptors type A
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-06-07T11:52:02Z
dc.journal.volume
127
dc.journal.number
1-2
dc.journal.pagination
31-37
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Puente, M. A.. Universidad Nacional de Lomas de Zamora; Argentina
dc.description.fil
Fil: Tartaglione, C. M.. Universidad Nacional de Lomas de Zamora; Argentina
dc.description.fil
Fil: Ritta, Monica Nora. Universidad Nacional de Lomas de Zamora; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Animal Reproduction Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432011001758
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.anireprosci.2011.07.001
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