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dc.contributor.author
Loera Quesada, Maribel
dc.contributor.author
Leyva González, Marco Antonio
dc.contributor.author
Velázquez Juárez, Gilberto
dc.contributor.author
Sánchez Calderón, Lenín
dc.contributor.author
Do Nascimento, Mauro
dc.contributor.author
López Arredondo, Damar
dc.contributor.author
Herrera Estrella, Luis
dc.date.available
2017-07-10T15:08:05Z
dc.date.issued
2016-03
dc.identifier.citation
Loera Quesada, Maribel; Leyva González, Marco Antonio; Velázquez Juárez, Gilberto; Sánchez Calderón, Lenín; Do Nascimento, Mauro; et al.; A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 14; 10; 3-2016; 2066-2076
dc.identifier.issn
1467-7644
dc.identifier.uri
http://hdl.handle.net/11336/19974
dc.description.abstract
Microalgal cultivation that takes advantage of solar energy is one of the most cost-effective systems for the biotechnological production of biofuels, and a range of high value products, including pharmaceuticals, fertilizers and feed. However, one of the main constraints for the cultivation of microalgae is the potential contamination with biological pollutants, such as bacteria, fungi, zooplankton or other undesirable microalgae. In closed bioreactors, the control of contamination requires the sterilization of the media, containers and all materials, which increases the cost of production, whereas open pond systems severely limits the number of species that can be cultivated under extreme environmental conditions to prevent contaminations. Here, we report the metabolic engineering of Chlamydomonas reinhardtii to use phosphite as its sole phosphorus source by expressing the ptxD gene from Pseudomonas stutzeri WM88, which encodes a phosphite oxidoreductase able to oxidize phosphite into phosphate using NAD as a cofactor. Engineered C. reinhardtii lines are capable of becoming the dominant species in a mixed culture when fertilized with phosphite as a sole phosphorus source. Our results represent a new platform for the production of microalgae, potentially useful for both closed photobioreactors and open pond systems without the need for using sterile conditions nor antibiotics or herbicides to prevent contamination with biological pollutants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Microalgae
dc.subject
Ptxd
dc.subject
Contaminant Management
dc.subject
Biofuels
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
dc.subject.classification
Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-06-21T19:59:35Z
dc.journal.volume
14
dc.journal.number
10
dc.journal.pagination
2066-2076
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Loera Quesada, Maribel. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad de Genómica Avanzada. Laboratorio Nacional de Genómica para la Biodiversidad; México
dc.description.fil
Fil: Leyva González, Marco Antonio. StelaGenomics; México
dc.description.fil
Fil: Velázquez Juárez, Gilberto. StelaGenomics; México
dc.description.fil
Fil: Sánchez Calderón, Lenín. StelaGenomics; México
dc.description.fil
Fil: Do Nascimento, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
dc.description.fil
Fil: López Arredondo, Damar. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad de Genómica Avanzada. Laboratorio Nacional de Genómica para la Biodiversidad; México
dc.description.fil
Fil: Herrera Estrella, Luis. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad de Genómica Avanzada. Laboratorio Nacional de Genómica para la Biodiversidad; México
dc.journal.title
Plant Biotechnology Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/pbi.12564
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/pbi.12564/abstract


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