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dc.contributor.author Loera Quesada, Maribel
dc.contributor.author Leyva González, Marco Antonio
dc.contributor.author Velázquez Juárez, Gilberto
dc.contributor.author Sánchez Calderón, Lenín
dc.contributor.author Do Nascimento, Mauro
dc.contributor.author López Arredondo, Damar
dc.contributor.author Herrera Estrella, Luis
dc.date.available 2017-07-10T15:08:05Z
dc.date.issued 2016-03
dc.identifier.citation Loera Quesada, Maribel; Leyva González, Marco Antonio; Velázquez Juárez, Gilberto; Sánchez Calderón, Lenín; Do Nascimento, Mauro; et al.; A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 14; 10; 3-2016; 2066-2076
dc.identifier.issn 1467-7644
dc.identifier.uri http://hdl.handle.net/11336/19974
dc.description.abstract Microalgal cultivation that takes advantage of solar energy is one of the most cost-effective systems for the biotechnological production of biofuels, and a range of high value products, including pharmaceuticals, fertilizers and feed. However, one of the main constraints for the cultivation of microalgae is the potential contamination with biological pollutants, such as bacteria, fungi, zooplankton or other undesirable microalgae. In closed bioreactors, the control of contamination requires the sterilization of the media, containers and all materials, which increases the cost of production, whereas open pond systems severely limits the number of species that can be cultivated under extreme environmental conditions to prevent contaminations. Here, we report the metabolic engineering of Chlamydomonas reinhardtii to use phosphite as its sole phosphorus source by expressing the ptxD gene from Pseudomonas stutzeri WM88, which encodes a phosphite oxidoreductase able to oxidize phosphite into phosphate using NAD as a cofactor. Engineered C. reinhardtii lines are capable of becoming the dominant species in a mixed culture when fertilized with phosphite as a sole phosphorus source. Our results represent a new platform for the production of microalgae, potentially useful for both closed photobioreactors and open pond systems without the need for using sterile conditions nor antibiotics or herbicides to prevent contamination with biological pollutants.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Microalgae
dc.subject ptxd
dc.subject Contaminant management
dc.subject Biofuels
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
dc.subject.classification Biotecnología Industrial
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-06-21T19:59:35Z
dc.journal.volume 14
dc.journal.number 10
dc.journal.pagination 2066-2076
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Loera Quesada, Maribel. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad de Genómica Avanzada. Laboratorio Nacional de Genómica para la Biodiversidad; México
dc.description.fil Fil: Leyva González, Marco Antonio. StelaGenomics; México
dc.description.fil Fil: Velázquez Juárez, Gilberto. StelaGenomics; México
dc.description.fil Fil: Sánchez Calderón, Lenín. StelaGenomics; México
dc.description.fil Fil: Do Nascimento, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
dc.description.fil Fil: López Arredondo, Damar. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad de Genómica Avanzada. Laboratorio Nacional de Genómica para la Biodiversidad; México
dc.description.fil Fil: Herrera Estrella, Luis. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad de Genómica Avanzada. Laboratorio Nacional de Genómica para la Biodiversidad; México
dc.journal.title Plant Biotechnology Journal
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/pbi.12564
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/pbi.12564/abstract


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)