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dc.contributor.author Acevedo, Raúl Maximiliano
dc.contributor.author Maiale, Santiago Javier
dc.contributor.author Pessino, Silvina Claudia
dc.contributor.author Bottini, Ambrosio Ruben
dc.contributor.author Ruiz, Oscar Adolfo
dc.contributor.author Sansberro, Pedro Alfonso
dc.date.available 2017-07-07T20:32:37Z
dc.date.issued 2013-01
dc.identifier.citation Acevedo, Raúl Maximiliano; Maiale, Santiago Javier; Pessino, Silvina Claudia; Bottini, Ambrosio Ruben; Ruiz, Oscar Adolfo; et al.; A succinate dehydrogenase flavoprotein subunit-like transcript is upregulated in Ilex paraguariensis leaves in response to water deficit and abscisic acid; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology And Biochemistry; 65; 1-2013; 48-54
dc.identifier.issn 0981-9428
dc.identifier.uri http://hdl.handle.net/11336/19917
dc.description.abstract Ilex paraguariensis plants were subjected to progressive soil water deficit, and differential display (DD) was used to analyse gene expression in leaves to characterise physiological responses to mild and severe water deficits. A cDNA fragment showing strong homology with the flavoprotein subunit (SDH1) of succinate:ubiquinone oxidoreductase (succinate dehydrogenase, SDH, EC 1.3.5.1) was upregulated in plants exposed to drought. Quantitative real-time PCR revealed that the SDH1-like transcript level began to increase when the leaf relative water content (RWC) decreased to 78% and peaked when the RWC dropped to 57%. A correlation between abscisic acid (ABA) concentration and variations in transcript levels was assessed by GC-SIM. After rehydration, SDH1 mRNA and ABA returned to their initial levels. In stressed leaves sprayed with ABA SDH1 mRNA accumulated in greater levels compared to stressed leaves that did not receive ABA. Moreover, the enzymatic activity of succinate dehydrogenase increased 1.5-fold in the mature leaves of ABA-treated plants. This physiological response may be related to the tendency of this species to minimise water losses through stomatal closure in the early stages of dehydration to avoid tissue desiccation. As the leaf water potential diminished due to an increase in water restriction, I. paraguariensis leaf tissues reacted by making osmotic adjustments to sustain tissue metabolic activity, which enables the recovery of photosynthesis upon re-watering. These results provide new insights concerning the linkage between plant respiration and photosynthetic metabolism that could be potentially further used in breeding programs aiming water tolerant genotypes.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier France-editions Scientifiques Medicales Elsevier
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Estres hidrico
dc.subject Yerba mate
dc.subject SDH1
dc.subject acido absisico
dc.subject.classification Agricultura
dc.subject.classification Agricultura, Silvicultura y Pesca
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title A succinate dehydrogenase flavoprotein subunit-like transcript is upregulated in Ilex paraguariensis leaves in response to water deficit and abscisic acid
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-06-08T19:48:56Z
dc.journal.volume 65
dc.journal.pagination 48-54
dc.journal.pais Francia
dc.journal.ciudad Paris
dc.description.fil Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Bottini, Ambrosio Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.fil Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.journal.title Plant Physiology And Biochemistry
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813000065
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2012.12.016


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)