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dc.contributor.author
Pisano, María Belén  
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Sánchez Seco, María Paz  
dc.contributor.author
Ré, Viviana Elizabeth  
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Farías, Adrián A.  
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Contigiani, Marta Silvia  
dc.contributor.author
Tenorio, Antonio  
dc.date.available
2023-05-31T14:44:41Z  
dc.date.issued
2012-05  
dc.identifier.citation
Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-206  
dc.identifier.issn
0166-0934  
dc.identifier.uri
http://hdl.handle.net/11336/199136  
dc.description.abstract
Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ALPHAVIRUS  
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ARBOVIRUS  
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DIAGNOSTIC  
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RT-NESTED PCR  
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SURVEILLANCE  
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VEEV  
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Biotecnología relacionada con la Salud  
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Biotecnología de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-05-30T11:50:05Z  
dc.journal.volume
186  
dc.journal.number
1-2  
dc.journal.pagination
203-206  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
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Fil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; España  
dc.description.fil
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.description.fil
Fil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina  
dc.description.fil
Fil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina  
dc.description.fil
Fil: Tenorio, Antonio. Instituto de Salud Carlos III; España  
dc.journal.title
Journal of Virological Methods  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093412001656  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jviromet.2012.05.009