Bioreactor treatment of aromatic hydrocarbons by indigenous micoflora and Gliocladium viride

Abstract

The PAH remediation depend on the bioavailability, aqueous solubility and high soil–water ratios that promote their accumulation in terrestrial and aquatic environments. Pollutant biodetoxification can be enhanced by bioaugmentation of degraders microorganism that produced biosurfactants. Therefore, the aims of this study were to assess the degradation potential of fluoranthene, pyrene and chrysene by filamentous fungi and to compare the detoxification ability of the indigenous fungi with isolated strains. A bioslurry reactors was implemented to evaluate the wild micoflora, Gliocladium viride and the combination of both ability to transformed hydrocarbons. A conspicuous transformation was obtained for fluorantene by the three communities, pyrene was degraded with a minor rate and chrysene was the most recalcitrant substrate. Unless fluorene, G. viride showed less uptake capacity for pyrene and chrysene; this two aromatics were significantly transformed by the wild fungi and the mixed culture. The PAHs concentrations by the three assays decreased 30 up to 70 % of the original levels. The PAH transformation by wild microflora and G. viride was significant increased in the bioreactor, due to the higher ratio water-solid the agitation kept the heterogeneous solids and PAHs in suspension and increased the aeration rates.