Artículo
Ultradeformable archaeosomes as new topical adjuvants
Higa, Leticia Herminia
; Schilrreff, Priscila
; Perez, Ana Paula
; Iriarte, Maiara A.; Roncaglia, Diana Inés; Morilla, María José
; Romero, Eder Lilia
Fecha de publicación:
11/2012
Editorial:
Elsevier Science
Revista:
Nanomedicine-nanotechnology Biology And Medicine
ISSN:
1549-9634
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Ultradeformable archaeosomes (UDA) are vesicles made of soybean phosphatidylcholine (SPC), sodium cholate (NaChol) and polar lipids from Halorubrum tebenquichense (3:1:3 wt/wt). Although ultradeformable liposomes (UDL, made of SPC and NaChol at 6:1 wt/wt) and UDA were neither captured nor caused cytotoxicity on keratinocytes, UDA was avidly captured by macrophages, their viability being reduced by 0.4-1.6 mg/mL phospholipids by 25 to 60%. Instead, UDL were poorly captured and caused no toxicity. Balb/C mice immunized by the topical route with four doses of ovalbumin (OVA)-loaded UDA, at 75 μg OVA/600 μg phospholipids (125 nm mean size and -42 mV zeta potential), induced IgG titers tenfold to 100-fold higher than those immunized with OVA-loaded UDL at the same dosage. Both matrices penetrate to the same skin depth (nearly 10 μm after 1 hour on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content.
Palabras clave:
SKIN
,
TOPICAL ADJUVANTS
,
VESICLES
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Higa, Leticia Herminia; Schilrreff, Priscila; Perez, Ana Paula; Iriarte, Maiara A.; Roncaglia, Diana Inés; et al.; Ultradeformable archaeosomes as new topical adjuvants; Elsevier Science; Nanomedicine-nanotechnology Biology And Medicine; 8; 8; 11-2012; 1319-1328
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