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dc.contributor.author
Raimunda, Daniel Cesar  
dc.contributor.author
Khare, Tripti  
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Giometti, Carol  
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Vogt, Stefan  
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Argüello, José M.  
dc.contributor.author
Finney, Lydia  
dc.date.available
2023-05-19T14:32:35Z  
dc.date.issued
2012-08  
dc.identifier.citation
Raimunda, Daniel Cesar; Khare, Tripti; Giometti, Carol; Vogt, Stefan; Argüello, José M.; et al.; Identifying metalloproteins through X-ray fluorescence mapping and mass spectrometry; Royal Society of Chemistry; Metallomics; 4; 9; 8-2012; 921-927  
dc.identifier.issn
1756-5901  
dc.identifier.uri
http://hdl.handle.net/11336/198114  
dc.description.abstract
Metals are critical and dynamic components of biochemistry. To understand their roles, we greatly need tools to identify the ligands that bind them within the complexity of natural systems. This work describes the development of methods that not only detect and distinguish metals, but also characterize the proteins that bind them. We describe an approach to expose, identify and quantify metalloproteins in complex mixtures by sequential non-denaturing 2D-gel electrophoresis (2D GE)/X-ray Fluorescence (XRF) and tandem mass spectrometry (MS/MS) in the same spot of a sample. We first apply the development of 2D GE/XRF to Shewanella oneidensis MR-1, a well-studied system, and verify our electrophoretic approach. Then, we identified a novel periplasmic zinc protein in Pseudomonas aeruginosa PAO1 through 2D GE/XRF followed by MS/MS. The identity and function of this protein was verified through a gene mutation experiment.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Royal Society of Chemistry  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Metalloproteins  
dc.subject
X-ray  
dc.subject
MS/MS  
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Fluorescence  
dc.subject.classification
Métodos de Investigación en Bioquímica  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Identifying metalloproteins through X-ray fluorescence mapping and mass spectrometry  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-05-18T14:35:14Z  
dc.identifier.eissn
1756-591X  
dc.journal.volume
4  
dc.journal.number
9  
dc.journal.pagination
921-927  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Cambridge  
dc.description.fil
Fil: Raimunda, Daniel Cesar. Worcester Polytechnic Institute; Estados Unidos  
dc.description.fil
Fil: Khare, Tripti. Argonne National Laboratory; Estados Unidos  
dc.description.fil
Fil: Giometti, Carol. Argonne National Laboratory; Estados Unidos  
dc.description.fil
Fil: Vogt, Stefan. Argonne National Laboratory; Estados Unidos  
dc.description.fil
Fil: Argüello, José M.. Worcester Polytechnic Institute; Estados Unidos  
dc.description.fil
Fil: Finney, Lydia. Argonne National Laboratory; Estados Unidos  
dc.journal.title
Metallomics  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/c2mt20095c  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/metallomics/article/4/9/921/6016121