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dc.contributor.author
Passone, María Alejandra
dc.contributor.author
Rosso, Laura Cristina
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dc.contributor.author
Etcheverry, Miriam Graciela
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dc.date.available
2023-05-18T15:04:04Z
dc.date.issued
2012-09
dc.identifier.citation
Passone, María Alejandra; Rosso, Laura Cristina; Etcheverry, Miriam Graciela; Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108; Elsevier Gmbh; Microbiological Research; 167; 8; 9-2012; 470-477
dc.identifier.issn
0944-5013
dc.identifier.uri
http://hdl.handle.net/11336/198038
dc.description.abstract
Effects of interacting conditions of sub-lethal levels of antioxidants, water potential (Ψ) and temperature were evaluated on growth, sclerotial characteristics, aflatoxin B 1 (AFB 1) production and aflD (=nor-1) gene expression by Aspergillus flavus strain RCP08108. These studies were carried out on peanut meal extract agar osmotically modified to -2.8,-7.1, -9.9 and -16.0MPa and incubated at 28 and 20°C. The food grade antioxidants added were butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) at (1+1mM-M1) and (5+5mM-M2). To relate the aflD expression after toxigenic A. flavus grew under interacting stress conditions, real-time PCR was used. Antioxidant mixtures caused a higher and significant (p<0.001) reduction in growth rate. The major impact on size and volume sclerotia was produced by Ψ; followed by antioxidant mixtures. High AFB 1 levels were observed in response to the M1 applied at -7.1MPa. Induction of the aflD gene was observed in response to the M1 treatment at -2.8, -7.1 and -9.9MPa; but significant decreases of AFB 1 production and aflD transcripts were observed; when the fungus grew in the presence of the M2 treatment.These results showed that it is necessary to apply food-grade antioxidants into the peanut storage system at levels higher than 5mM. This is an important tool to avoid sub-lethal antioxidant doses that can lead to fungal growth, increase resistance structures, and stimulate aflD gene expression and AFB 1 accumulation in this substrate.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Gmbh
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AFLATOXINS
dc.subject
ANTIOXIDANTS
dc.subject
ASPERGILLUS FLAVUS
dc.subject
ENVIRONMENTAL FACTORS
dc.subject
GROWTH
dc.subject
SCLEROTIA
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-05-18T14:05:14Z
dc.journal.volume
167
dc.journal.number
8
dc.journal.pagination
470-477
dc.journal.pais
Alemania
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dc.description.fil
Fil: Passone, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina
dc.description.fil
Fil: Rosso, Laura Cristina. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.journal.title
Microbiological Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0944501311001030
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.micres.2011.11.004
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