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dc.contributor.author
Bourguignon, Natalia  
dc.contributor.author
Perez, Maximiliano Sebastian  
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Pérez Sosa, Camilo José  
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Aravelli, Aparna  
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Bravo, Elisa  
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Perez, Maximiliano Sebastian  
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Miriuka, Santiago Gabriel  
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Lerner, Betiana  
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Bhansali, Shekhar  
dc.date.available
2023-05-18T12:57:44Z  
dc.date.issued
2022-10  
dc.identifier.citation
Bourguignon, Natalia; Perez, Maximiliano Sebastian; Pérez Sosa, Camilo José; Aravelli, Aparna; Bravo, Elisa; et al.; Micro and milli-chamber bioreactors for human induced pluripotent stem cell culture: Model and experimental validation; Elsevier Science SA; Biochemical Engineering Journal; 187; 10-2022; 1-8  
dc.identifier.issn
1369-703X  
dc.identifier.uri
http://hdl.handle.net/11336/197969  
dc.description.abstract
One of the main interests in developing microfluidic platforms is the lab-on-a-chip (LOC) applications, including the use of microdevices for the culture of prokaryotic and eukaryotic cells for different applications. In this context, the trapping and control of Micro/nano-scale bubbles have been considered as it becomes a field of growing concern addressing many challenges in LOC applications. In the present work, we present a novel microfluidic bioreactor based on the micropillar design that reduces stresses caused by bubbles on the cell growth in the microchip. The combination of one chamber with 3-pillars and 10-pillars design with the channel heights of 270 µm and 2 mm were evaluated. Computational fluid dynamics simulations (CFD) were conducted to investigate the flow dynamics in the new designs. Numerical values of velocity were compared with those obtained by experimental flow tests to evaluate the performance of the proposed microdevice. Finally, the functionality of the new milli-bioreactor design was validated by culturing human induced pluripotent stem cells (hiPSC) for 5 days. Cell growth, viability and sustain pluripotency were successfully proved using a new platform architecture. This multidisciplinary research provides new insights into the chemical engineering and operability of micro and milli-bioreactors with prospects for future industrial applications.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science SA  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
BUBBLES CONTROL  
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COMPUTATIONAL FLUID DYNAMICS  
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HIPSC  
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MICROPILLARS  
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MILLIBIOREACTOR  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Micro and milli-chamber bioreactors for human induced pluripotent stem cell culture: Model and experimental validation  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-05-16T11:24:48Z  
dc.journal.volume
187  
dc.journal.pagination
1-8  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Perez, Maximiliano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Pérez Sosa, Camilo José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Aravelli, Aparna. Florida International University; Estados Unidos  
dc.description.fil
Fil: Bravo, Elisa. Florida International University; Estados Unidos  
dc.description.fil
Fil: Perez, Maximiliano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Bhansali, Shekhar. Florida International University; Estados Unidos  
dc.journal.title
Biochemical Engineering Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.bej.2022.108660