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dc.contributor.author
Miranda-Miranda, Estefan
dc.contributor.author
Scarcella, Silvana Andrea
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Reynaud, Enrique
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Narváez Padilla, Verónica
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Neira, Gisela Natalia
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Mera y Sierra, Roberto
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Aguilar Díaz, Hugo
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Cossio Bayugar, Raquel
dc.date.available
2023-05-17T17:58:37Z
dc.date.issued
2022-10
dc.identifier.citation
Miranda-Miranda, Estefan; Scarcella, Silvana Andrea; Reynaud, Enrique; Narváez Padilla, Verónica; Neira, Gisela Natalia; et al.; A Single Nucleotide Polymorphism Translates into a Radical Amino Acid Substitution at the Ligand-Binding Site in Fasciola hepatica Carboxylesterase B; MDPI; Genes; 13; 10; 10-2022; 1-11
dc.identifier.issn
2073-4425
dc.identifier.uri
http://hdl.handle.net/11336/197834
dc.description.abstract
Fasciola hepatica anthelmintic resistance may be associated with the catalytic activity of xenobiotic metabolizing enzymes. The gene expression of one of these enzymes, identified as carboxylesterase B (CestB), was previously described as inducible in adult parasites under anthelmintic treatment and exhibited a single nucleotide polymorphism at position 643 that translates into a radical amino acid substitution at position 215 from Glutamic acid to Lysine. Alphafold 3D models of both allelic sequences exhibited a significant affinity pocket rearrangement and different ligand-docking modeling results. Further bioinformatics analysis confirmed that the radical amino acid substitution is located at the ligand affinity site of the enzyme, affecting its affinity to serine hydrolase inhibitors and preferences for ester ligands. A field genotyping survey from parasite samples obtained from two developmental stages isolated from different host species from Argentina and Mexico exhibited a 37% allele distribution for 215E and a 29% allele distribution for 215K as well as a 34% E/K heterozygous distribution. No linkage to host species or geographic origin was found in any of the allele variants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
AMINO ACID SUBSTITUTION
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ANTHELMINTIC RESISTANCE
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BIOINFORMATICS
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CARBOXYLESTERASE
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FASCIOLA HEPATICA
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SNTP
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A Single Nucleotide Polymorphism Translates into a Radical Amino Acid Substitution at the Ligand-Binding Site in Fasciola hepatica Carboxylesterase B
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-05-17T15:21:12Z
dc.journal.volume
13
dc.journal.number
10
dc.journal.pagination
1-11
dc.journal.pais
Reino Unido
dc.journal.ciudad
York
dc.description.fil
Fil: Miranda-Miranda, Estefan. Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias; México
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Fil: Scarcella, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
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Fil: Reynaud, Enrique. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Narváez Padilla, Verónica. Universidad Autónoma del Estado de Morelos; México
dc.description.fil
Fil: Neira, Gisela Natalia. Universidad "Juan Agustín Maza". Facultad de Ciencias Veterinarias y Ambientales. Centro de Investigación en Parasitología Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
dc.description.fil
Fil: Mera y Sierra, Roberto. Universidad "Juan Agustín Maza". Facultad de Ciencias Veterinarias y Ambientales. Centro de Investigación en Parasitología Regional; Argentina
dc.description.fil
Fil: Aguilar Díaz, Hugo. Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias; México
dc.description.fil
Fil: Cossio Bayugar, Raquel. Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias; México
dc.journal.title
Genes
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/genes13101899
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