Artículo
TGF-β neutralization abrogates the inhibited DHEA production mediated by factors released from M. tuberculosis-stimulated PBMC
D'attilio, Luciano David
; Bozza, Verónica Vanina
; Santucci, Natalia Estefanía
; Bongiovanni, Bettina
; Dídoli, Griselda; Radcliffe, Stella; Besedovsky, Hugo; del Rey, Adriana; Bottasso, Oscar Adelmo
; Bay, Maria Luisa
Fecha de publicación:
07/2012
Editorial:
Blackwell Publishing
Revista:
Annals of the New York Academy of Sciences
ISSN:
0077-8923
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Supernatants (SN) from cultures of peripheral blood mononuclear cells (PBMC) of tuberculosis (TB) patients inhibit dehydroepiandrosterone (DHEA) secretion by the adrenal cell line NCI-H295R. To analyze whether TGF-β is involved in this effect, SN of PBMC from healthy controls or patients with severe TB infections, stimulated or not with Mycobacterium tuberculosis (Mtb SN), were added to adrenal cells under basal conditions or following stimulation with forskolin. Cortisol and DHEA concentrations were evaluated in supernatants of the adrenal cells cultured with or without the addition of anti-TGF-β. Treatment with Mtb SN from TB inhibited DHEA production, and this effect was reversed when SN were treated with anti-TGF-β. The increase in cortisol production induced by SN from TB patients was not affected by TGF-β neutralization. Mediators released during the anti-TB immune response differentially modulate steroid production by adrenal cells, and TGF-β is a cytokine implicated in the inhibition of DHEA production observed in TB.
Palabras clave:
DEHYDROEPIANDROSTERONE
,
CORTISOL
,
TGF-BETA
,
NCI-H295R CELL LINE
,
TUBERCULOSIS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
D'attilio, Luciano David; Bozza, Verónica Vanina; Santucci, Natalia Estefanía; Bongiovanni, Bettina; Dídoli, Griselda; et al.; TGF-β neutralization abrogates the inhibited DHEA production mediated by factors released from M. tuberculosis-stimulated PBMC; Blackwell Publishing; Annals of the New York Academy of Sciences; 1262; 1; 7-2012; 1-9
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