Artículo
Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides
Moyano, Ana L.; Comin, Romina
; Vilcaes, Aldo Alejandro
; Funes, Samanta Celeste
; Roth, German Alfredo
; Irazoqui, Fernando Jose
; Nores, Gustavo Alejandro
Fecha de publicación:
12/2012
Editorial:
Oxford Univ Press Inc
Revista:
Glycobiology
ISSN:
0959-6658
e-ISSN:
1460-2423
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed density-dependent binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).
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Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Moyano, Ana L.; Comin, Romina; Vilcaes, Aldo Alejandro; Funes, Samanta Celeste; Roth, German Alfredo; et al.; Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides; Oxford Univ Press Inc; Glycobiology; 22; 12; 12-2012; 1768-1774
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