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dc.contributor.author
Sturgill, Elizabeth R.
dc.contributor.author
Aoki, Kazuhiro
dc.contributor.author
Lopez, Pablo
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dc.contributor.author
Colacurcio, Daniel
dc.contributor.author
Vajn, Katarina
dc.contributor.author
Lorenzini, Ileana
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Majic, Senka
dc.contributor.author
Yang, Won Ho
dc.contributor.author
Heffer, Marija
dc.contributor.author
Tiemeyer, Michael
dc.contributor.author
Marth, Jamey D.
dc.contributor.author
Schnaar, Ronald L.
dc.date.available
2023-05-15T16:54:06Z
dc.date.issued
2012-10
dc.identifier.citation
Sturgill, Elizabeth R.; Aoki, Kazuhiro; Lopez, Pablo; Colacurcio, Daniel; Vajn, Katarina; et al.; Biosynthesis of the major brain gangliosides GD1a and GT1b; Oxford Univ Press Inc; Glycobiology; 22; 10; 10-2012; 1289-1301
dc.identifier.issn
0959-6658
dc.identifier.uri
http://hdl.handle.net/11336/197563
dc.description.abstract
Gangliosides-sialylated glycosphingolipids-are the major glycoconjugates of nerve cells. The same four structures-GM1, GD1a, GD1b and GT1b-comprise the great majority of gangliosides in mammalian brains. They share a common tetrasaccharide core (Gal1-3GalNAc1-4Gal1-4Glc1-1′Cer) with one or two sialic acids on the internal galactose and zero (GM1 and GD1b) or one (GD1a and GT1b) 2-3-linked sialic acid on the terminal galactose. Whereas the genes responsible for the sialylation of the internal galactose are known, those responsible for terminal sialylation have not been established in vivo. We report that St3gal2 and St3gal3 are responsible for nearly all the terminal sialylation of brain gangliosides in the mouse. When brain ganglioside expression was analyzed in adult St3gal1-, St3gal2-, St3gal3-and St3gal4-null mice, only St3gal2-null mice differed significantly from wild type, expressing half the normal amount of GD1a and GT1b. St3gal1/2-double-null mice were no different than St3gal2-single-null mice; however, St3gal2/3-double-null mice were >95 depleted in gangliosides GD1a and GT1b. Total ganglioside expression (lipid-bound sialic acid) in the brains of St3gal2/3-double-null mice was equivalent to that in wild-type mice, whereas total protein sialylation was reduced by half. St3gal2/3-double-null mice were small, weak and short lived. They were half the weight of wild-type mice at weaning and displayed early hindlimb dysreflexia. We conclude that the St3gal2 and St3gal3 gene products (ST3Gal-II and ST3Gal-III sialyltransferases) are largely responsible for ganglioside terminal 2-3 sialylation in the brain, synthesizing the major brain gangliosides GD1a and GT1b. © 2012 The Author.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford Univ Press Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BRAIN
dc.subject
GANGLIOSIDE
dc.subject
MYELIN
dc.subject
SIALIC ACID
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SIALYLTRANSFERASE
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Biosynthesis of the major brain gangliosides GD1a and GT1b
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-05-12T10:19:57Z
dc.identifier.eissn
1460-2423
dc.journal.volume
22
dc.journal.number
10
dc.journal.pagination
1289-1301
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Oxford
dc.description.fil
Fil: Sturgill, Elizabeth R.. Johns Hopkins School Of Medicine; Estados Unidos. University Johns Hopkins; Estados Unidos
dc.description.fil
Fil: Aoki, Kazuhiro. University of Georgia; Estados Unidos
dc.description.fil
Fil: Lopez, Pablo. University Johns Hopkins; Estados Unidos
dc.description.fil
Fil: Colacurcio, Daniel. University Johns Hopkins; Estados Unidos
dc.description.fil
Fil: Vajn, Katarina. University Johns Hopkins; Estados Unidos
dc.description.fil
Fil: Lorenzini, Ileana. University Johns Hopkins; Estados Unidos
dc.description.fil
Fil: Majic, Senka. University Johns Hopkins; Estados Unidos
dc.description.fil
Fil: Yang, Won Ho. University of California; Estados Unidos
dc.description.fil
Fil: Heffer, Marija. J. J. Strossmayer University; Croacia
dc.description.fil
Fil: Tiemeyer, Michael. University of Georgia; Estados Unidos
dc.description.fil
Fil: Marth, Jamey D.. University of California; Estados Unidos
dc.description.fil
Fil: Schnaar, Ronald L.. University Johns Hopkins; Estados Unidos
dc.journal.title
Glycobiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/glycob/cws103
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article/22/10/1289/1988226
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