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dc.contributor.author
Martinez, Maria Julia  
dc.contributor.author
Andreu, Adriana Balbina  
dc.contributor.author
Barbini, Luciana Fernanda  
dc.date.available
2023-05-11T14:27:03Z  
dc.date.issued
2022-12  
dc.identifier.citation
Martinez, Maria Julia; Andreu, Adriana Balbina; Barbini, Luciana Fernanda; Cytotoxic activity of Solanum tuberosum polyphenolic extracts in human hepatocarcinoma cells is mediated by apoptosis and autophagy; Wiley Blackwell Publishing, Inc; Journal of Food Science; 87; 12; 12-2022; 5303-5316  
dc.identifier.issn
0022-1147  
dc.identifier.uri
http://hdl.handle.net/11336/197210  
dc.description.abstract
Hepatocellular carcinoma (HCC) is one of the most frequent tumors worldwide, associated with a high mortality rate. HCC patient´s prognosis remains unfavorable, mainly due to late diagnosis and treatment instauration delay. Thus, there is an urgent requirement for the development of new therapeutical options, particularly for advanced HCC patients. Polyphenols are specialized plant metabolites, which have been broadly characterized for their beneficial effects on human health. Potato is one of the main sources of these compounds in human diet, due to its high consumption. The objectives of this study were to: characterize the polyphenolic composition, analyze the antioxidant properties, evaluate the cytotoxic activity in human tumoral hepatocytes, and describe the molecular mechanisms involved in cell death, of potato polyphenolic extracts (PPEs) from the pigmented Andigena cultivar CL658. The results showed that phenolic acids were the main group of polyphenols in the extracts, chlorogenic acid being the major compound, followed by caffeic acid. HPLC-DAD profile showed pelargonidin as the principal anthocyanin. The treatment of HCC cell lines with the cytotoxic concentration 50 (CC50 ) of the PPEs resulted in cytotoxicity, evidenced by increases in the percentage of dead cells (5-10%) after treatments. This cytotoxic effect was mainly due to apoptosis activation, evidenced by modulation of the Bcl-2 family proteins expression, in favor of pro-apoptotic members over anti-apoptotic in the final balance. PPEs also induced autophagy, indicated by increased LC3 expression (122-274%) and autophagosomes, and reduced p62 expression (32-48%), in variable magnitudes according to the treated cell line. Overall, these results support the potential use of PPEs from the cultivar CL658 as a source of bioactive compounds for novel treatments against HCC.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ANTIOXIDANT  
dc.subject
APOPTOSIS  
dc.subject
CYTOTOXICITY  
dc.subject
POLYPHENOLS  
dc.subject.classification
Fisiología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Cytotoxic activity of Solanum tuberosum polyphenolic extracts in human hepatocarcinoma cells is mediated by apoptosis and autophagy  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-05-11T14:11:00Z  
dc.journal.volume
87  
dc.journal.number
12  
dc.journal.pagination
5303-5316  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Martinez, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Barbini, Luciana Fernanda. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina  
dc.journal.title
Journal of Food Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/1750-3841.16366