Artículo
Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum
Olson, Linda J.; Orsi, Ramiro
; Alculumbre, Solana G.; Peterson, Francis C.; Stigliano, Ivan Daniel
; Parodi, Armando Jose A.
; D'alessio, Cecilia
; Dahms, Nancy M.
Fecha de publicación:
06/2013
Editorial:
American Society for Biochemistry and Molecular Biology
Revista:
Journal Of Biological Chemistry (online)
ISSN:
0021-9258
e-ISSN:
1083-351X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Here we report for the first time the three-dimensional structure of a mannose 6-phosphate receptor homology (MRH) domain present in a protein with enzymatic activity, glucosidase II (GII). GII is involved in glycoprotein folding in the endoplasmic reticulum. GII removes the two innermost glucose residues from the Glc3Man9GlcNAc2 transferred to nascent proteins and the glucose added by UDP-Glc:glycoprotein glucosyltransferase. GII is composed of a catalytic GIIα subunit and a regulatory GIIβ subunit. GIIβ participates in the endoplasmic reticulum localization of GIIα and mediates in vivo enhancement of N-glycan trimming by GII through its C-terminal MRH domain. We determined the structure of a functional GIIβ MRH domain by NMR spectroscopy. It adopts a β-barrel fold similar to that of other MRH domains, but its binding pocket is the most shallow known to date as it accommodates a single mannose residue. In addition, we identified a conserved residue outside the binding pocket (Trp-409) present in GIIβ but not in other MRHs that influences GII glucose trimming activity.
Palabras clave:
Endoplasmic Reticulum
,
Glucosidase Ii B
,
Glycobiology
,
Mrh Domain
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Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Olson, Linda J.; Orsi, Ramiro; Alculumbre, Solana G.; Peterson, Francis C.; Stigliano, Ivan Daniel; et al.; Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum; American Society for Biochemistry and Molecular Biology; Journal Of Biological Chemistry (online); 288; 23; 6-2013; 16460-16475
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