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dc.contributor.author
Martínez, Fernando Gabriel
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dc.contributor.author
Moreno Martin, Gustavo
dc.contributor.author
Madrid Albarrán, Yolanda
dc.contributor.author
Ordoñez, Omar Federico
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Pescuma, Micaela
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dc.contributor.author
Mozzi, Fernanda Beatriz
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dc.date.available
2023-05-10T20:19:33Z
dc.date.issued
2021
dc.identifier.citation
Functional fermented beberages enriched in seleno-amino acids and seleno-nanoparticles; LVI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XV Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2020; 78-78
dc.identifier.issn
0327-9545
dc.identifier.uri
http://hdl.handle.net/11336/197086
dc.description.abstract
Selenium (Se) is an essential micronutrient for human health, which is found as selenocysteine (SeCys) in the active site of Se-dependent enzymes involved in the response to oxidative stress and in thyroid functions. The main inorganic Se forms, selenite and selenate are toxic. Some lactic acid bacteria (LAB) can reduce Se salts into seleno-nanoparticles (SeNPs) and seleno-amino acids, which are non-toxic and highly bioavailable forms. In several European countries, as well as in Argentina, Se intake is below the recommended dietary intake (RDI). Se-enrichment of foods is an attractive strategy to increase its ingestion. We aimed to formulate a fermented fruit juice-milk beverage (FJMB) bio-enriched in Se. The fruit-origin strains Fructobacillus tropaeoli CRL 2034 and Levilactobacillus brevis CRL 2051 were grown with or without 5 mg/L of Se prior to co-inoculation (1% of each strain) in the FJMB and were incubated 14 h at 30 °C. The survival of the strains under storage conditions (6 °C, 52 days), and after digestion [using an in vitro gastrointestinal system (GIS)] was analyzed. The strains grew (up to 8.6 U log each) and acidified FJMB reaching a final pH of 4.6. Sugar metabolism and organic acid production were similar for control and selenized cells (RP-HPLC); while mannitol production by selenized cells of the Fructobacillus was lower (0.18 ± 0.03) than control cells. The studied strains could not degrade the proteins present in the FJMB (SDS-PAGE). Selenized cells increased the beverage total Se concentration (ICP-MS, 84.9 ± 4.5 μg/L) and biotransformed selenite into SeCys (39.1 ± 0.4 μg/L) and SeMet (6.1 ± 0.1 μg/L) as detected by LC-ICP-MS. Moreover, SEM images of the fermented FJMB revealed the presence of SeNPs attached to the cell surface of both strains. Interestingly, microbial resistance at the end of the shelf life was greater (between 0.5 and 0.7 U log) for selenized than non-selenized cells. However, no differences were observed in the sugar and organic acid concentrations between treated and non-treated cells and a lower (0.29 ± 0.04 g/L) mannitol production was detected at 28 day incubation by the treated strains. After GIS digestion, a decrease in the cell counts of F. tropaeoli and L. brevis (1.60 and 0.80 U log, respectively) was observed. Interestingly, 64.3 ± 3.3 μg total Se/L partly as SeCys (25.8 ± 2.3 μg/L) and SeMet (2.4 ± 0.2 μg/L) were found in the FJMB supernatant after intestinal digestion, highlighting the bioaccessibility of these compounds. Remarkably, 250 mL of the FJMB could cover 64% of the Se RDI (25 μg/day), from which 28% is composed by seleno-amino acids. Our results suggest that selenized cells of F. tropaeoli CRL 2034 and L. brevis CRL 2051 could be used for formulating functional Se-enriched beverages to improve this micronutrient intake in humans.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Tech Science Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
SELENIUM
dc.subject
SELENOCYSTEINE
dc.subject
LACTIC ACID BACTERIA
dc.subject
SELENO-NANOPARTICLES
dc.subject
SE-ENRICHMENT
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Functional fermented beberages enriched in seleno-amino acids and seleno-nanoparticles
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-01T15:31:24Z
dc.identifier.eissn
1667-5746
dc.journal.volume
47
dc.journal.number
Suppl. 1
dc.journal.pagination
78-78
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Henderson
dc.description.fil
Fil: Martínez, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
dc.description.fil
Fil: Moreno Martin, Gustavo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
dc.description.fil
Fil: Madrid Albarrán, Yolanda. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
dc.description.fil
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
dc.description.fil
Fil: Pescuma, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
dc.description.fil
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar//wp-content/uploads/2022/10/Libro-de-resumenes-2020-Biocell.pdf
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Autor
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Autor
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Autor
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Autor
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Autor
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Autor
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dc.coverage
Internacional
dc.type.subtype
Reunión
dc.description.nombreEvento
LVI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XV Annual Meeting of the Argentinean Society for General Microbiology
dc.date.evento
2020-11-02
dc.description.ciudadEvento
Virtual
dc.description.paisEvento
Argentina
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dc.type.publicacion
Journal
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General
dc.source.revista
Biocell
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dc.date.eventoHasta
2020-11-05
dc.type
Reunión
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