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dc.contributor.author
Prieto Gonzalez, Elio A.
dc.contributor.author
Mudry, Marta Dolores
dc.contributor.author
Palermo, Ana María
dc.date.available
2017-07-05T20:04:14Z
dc.date.issued
2014-11
dc.identifier.citation
Prieto Gonzalez, Elio A.; Mudry, Marta Dolores; Palermo, Ana María; DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects; Begell House Inc; Journal of Environmental Pathology, Toxicology and Oncology; 33; 4; 11-2014; 279-293
dc.identifier.issn
0731-8898
dc.identifier.uri
http://hdl.handle.net/11336/19668
dc.description.abstract
The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2 O2 (150 µM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2 O2 than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Begell House Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Comet Assay
dc.subject
Dna Repair Mechanisms
dc.subject
Base Excision Repair
dc.subject
Nucleotide Excision Repair
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-05T15:02:44Z
dc.journal.volume
33
dc.journal.number
4
dc.journal.pagination
279-293
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Prieto Gonzalez, Elio A.. Universidad Abierta Interamericana; Argentina
dc.description.fil
Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
dc.description.fil
Fil: Palermo, Ana María. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas Para la Defensa; Argentina
dc.journal.title
Journal of Environmental Pathology, Toxicology and Oncology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,3d6bbf6e6e579abd,6591f2615d0ec268.html


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