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dc.contributor.author Prieto Gonzalez, Elio A.
dc.contributor.author Mudry, Marta Dolores
dc.contributor.author Palermo, Ana María
dc.date.available 2017-07-05T20:04:14Z
dc.date.issued 2014-11
dc.identifier.citation Prieto Gonzalez, Elio A.; Mudry, Marta Dolores; Palermo, Ana María; DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects; Begell House Inc; Journal of Environmental Pathology, Toxicology and Oncology; 33; 4; 11-2014; 279-293
dc.identifier.issn 0731-8898
dc.identifier.uri http://hdl.handle.net/11336/19668
dc.description.abstract The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2 O2 (150 µM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2 O2 than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring
dc.format application/pdf
dc.language.iso eng
dc.publisher Begell House Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject COMET ASSAY
dc.subject DNA REPAIR MECHANISMS
dc.subject BASE EXCISION REPAIR
dc.subject NUCLEOTIDE EXCISION REPAIR
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-05T15:02:44Z
dc.journal.volume 33
dc.journal.number 4
dc.journal.pagination 279-293
dc.journal.pais Estados Unidos
dc.description.fil Fil: Prieto Gonzalez, Elio A.. Universidad Abierta Interamericana; Argentina
dc.description.fil Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
dc.description.fil Fil: Palermo, Ana María. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas Para la Defensa; Argentina
dc.journal.title Journal of Environmental Pathology, Toxicology and Oncology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014008234
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,3d6bbf6e6e579abd,6591f2615d0ec268.html


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)