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dc.contributor.author
Crispo, Martina
dc.contributor.author
Van Maele, Laurye
dc.contributor.author
Tabareau, Julien
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Cayet, Delphine
dc.contributor.author
Errea, Agustina Juliana
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Ferreira, Ana María
dc.contributor.author
Rumbo, Martin
dc.contributor.author
Sirard, Jean Claude
dc.date.available
2017-07-05T19:45:34Z
dc.date.issued
2013-11-12
dc.identifier.citation
Crispo, Martina; Van Maele, Laurye; Tabareau, Julien; Cayet, Delphine; Errea, Agustina Juliana; et al.; Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response; Public Library Science; Plos One; 8; 11; 12-11-2013; 13-16
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/19660
dc.description.abstract
The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ccl20
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Luciferase Reporter
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Transgenic Mice
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Liver
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2015-05-20T18:18:24Z
dc.journal.volume
8
dc.journal.number
11
dc.journal.pagination
13-16
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Crispo, Martina. Unidad de Animales Transgénicos y de Experimentación – Institut Pasteur de Montevideo; Uruguay
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Fil: Van Maele, Laurye. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
dc.description.fil
Fil: Tabareau, Julien. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
dc.description.fil
Fil: Cayet, Delphine. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
dc.description.fil
Fil: Errea, Agustina Juliana. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; Argentina
dc.description.fil
Fil: Ferreira, Ana María. Cátedra de Inmunologíaa. Facultad de Ciencias/Facultad de Química. Universidad de la República; Uruguay
dc.description.fil
Fil: Rumbo, Martin. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune; Argentina
dc.description.fil
Fil: Sirard, Jean Claude. Institut Pasteur de Lille. Centre d’Infection et d’Immunite de Lille; Francia
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078447
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0078447
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