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dc.contributor.author
Fernandez, Juan Manuel
dc.contributor.author
Molinuevo, María Silvina
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dc.contributor.author
Cortizo, Maria Susana
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dc.contributor.author
McCarthy, Antonio Desmond
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dc.contributor.author
Cortizo, Ana María
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dc.date.available
2023-05-05T16:19:56Z
dc.date.issued
2011-06
dc.identifier.citation
Fernandez, Juan Manuel; Molinuevo, María Silvina; Cortizo, Maria Susana; McCarthy, Antonio Desmond; Cortizo, Ana María; Development of an osteoconductive PCL-PDIPF-hydroxyapatite composite scaffold for bone tissue engineering; John Wiley & Sons Ltd; Journal Of Tissue Engineering And Regenerative Medicine; 5; 6; 6-2011; 126-135
dc.identifier.issn
1932-6254
dc.identifier.uri
http://hdl.handle.net/11336/196488
dc.description.abstract
Hydroxyapatite (HAP)-containing poly-å-caprolactone (PCL)/ polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongation-at-break of Blend-HAP suggest that the mechanical properties of the HAP-scaffolds have improved significantly. The addition of HAP to both PCL and Blend, significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: a) HAP-Polymer increases proliferation of osteoblastic cells, b) HAP included in the blend increases the ALP expression in UMR106 cells, c) HAP-Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP-polymer scaffolds. In conclusion, a novel biocompatible HAP-Blend composite with uniform dispersion of semi-nano HAP particles and good inter-phase compatibility has been prepared successfully. The development of HAP-Blend composite, with improved physical, mechanical and osteoinductive properties may be potentially used in bone tissue engineering applications. fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongation-at-break of Blend-HAP suggest that the mechanical properties of the HAP-scaffolds have improved significantly. The addition of HAP to both PCL and Blend, significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: a) HAP-Polymer increases proliferation of osteoblastic cells, b) HAP included in the blend increases the ALP expression in UMR106 cells, c) HAP-Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP-polymer scaffolds. In conclusion, a novel biocompatible HAP-Blend composite with uniform dispersion of semi-nano HAP particles and good inter-phase compatibility has been prepared successfully. The development of HAP-Blend composite, with improved physical, mechanical and osteoinductive properties may be potentially used in bone tissue engineering applications. fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongation-at-break of Blend-HAP suggest that the mechanical properties of the HAP-scaffolds have improved significantly. The addition of HAP to both PCL and Blend, significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: a) HAP-Polymer increases proliferation of osteoblastic cells, b) HAP included in the blend increases the ALP expression in UMR106 cells, c) HAP-Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP-polymer scaffolds. In conclusion, a novel biocompatible HAP-Blend composite with uniform dispersion of semi-nano HAP particles and good inter-phase compatibility has been prepared successfully. The development of HAP-Blend composite, with improved physical, mechanical and osteoinductive properties may be potentially used in bone tissue engineering applications. å-caprolactone (PCL)/ polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongation-at-break of Blend-HAP suggest that the mechanical properties of the HAP-scaffolds have improved significantly. The addition of HAP to both PCL and Blend, significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: a) HAP-Polymer increases proliferation of osteoblastic cells, b) HAP included in the blend increases the ALP expression in UMR106 cells, c) HAP-Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP-polymer scaffolds. In conclusion, a novel biocompatible HAP-Blend composite with uniform dispersion of semi-nano HAP particles and good inter-phase compatibility has been prepared successfully. The development of HAP-Blend composite, with improved physical, mechanical and osteoinductive properties may be potentially used in bone tissue engineering applications.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOCOMPATIBILITY
dc.subject
BONE TISSUE ENGINEERING
dc.subject
HYDROXYAPATITE
dc.subject
OSTEOBLASTS
dc.subject
POLY-Ε-CAPROLACTONE
dc.subject
POLYDIALKYL FUMARATES
dc.subject.classification
Biomateriales
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dc.subject.classification
Biotecnología de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Development of an osteoconductive PCL-PDIPF-hydroxyapatite composite scaffold for bone tissue engineering
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-04-10T10:11:38Z
dc.journal.volume
5
dc.journal.number
6
dc.journal.pagination
126-135
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: Fernandez, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
dc.description.fil
Fil: Molinuevo, María Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.description.fil
Fil: Cortizo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
dc.description.fil
Fil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
dc.description.fil
Fil: Cortizo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
dc.journal.title
Journal Of Tissue Engineering And Regenerative Medicine
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/term.394
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/term.394
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