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Chapana, Agostina Lucía  
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Guarniolo, D.  
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Carvelli, Flavia Lorena  
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Sosa, E.  
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Fernández, R. A.  
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Sosa, M. A.  
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Caballero, P. A.  
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Piezzi, Ramon Salvador  
dc.date.available
2023-05-03T17:03:13Z  
dc.date.issued
2021  
dc.identifier.citation
Effect of botulinum neurotoxins from Mendoza of clostridium botulinum strains on cytoskeletal proteins of mammary tumor cells; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Argentina; 2020; 16-16  
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0327-9545  
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http://hdl.handle.net/11336/196118  
dc.description.abstract
The botulinum neurotoxin serotype A (BoNT A) produced by Clostridium botulinum, which causes botulism, is used for the treatment of multiple neurological diseases and its therapeutic action against cancer is currently being evaluated. In previous studies, we have shown that BoNT A from autochthonous soil strains (Su) have different properties than the reference A Hall strain. Among these, its molecular structure, its enzymatic activity against brain SNARE proteins and its greater specific toxic activity (AE) stand out. In cells from human mammary carcinoma (MCF-7) treated with BoNTs for 45 min, we found a marked effect on the expression of cytoskeletal proteins. Therefore, in this work, we delve into the study of the action of autochthonous BoNTs A and prototype A Hall on the distribution of actin and tubulin in these cells. Native forms of autochthonous BoNT (Su strains 1935 and 1891, Tupungato) and prototype A Hall were purified by saline precipitation. Their AE values (LD50 / mg protein) were established and their electrophoretic characteristics were evaluated under non-denaturing conditions. 250 LD50 of the BoNTs were incubated to MCF-7 cell cultures for 10 or 25 min. Later, the cells were fixed and processed for indirect immunofluorescence with the use of specific antibodies that recognize tubulin or actin. The samples were visualized by fluorescence microscopy. At the two times evaluated, the three types of BoNTs produced a marked redistribution of the actin cytoskeleton, patch form, on areas coinciding with the plasma membrane. Tubulin was redistributed to multiple areas with high signal density at 10 min of incubation only in the presence of BoNT 1891. At 25 min of incubation, the cells treated with BoNTs 1891 and 1935 showed this effect, while in those incubated with A Hall, the distribution of these proteins was not modified. The notable alterations in the distribution of components of the tumor cell cytoskeleton by BoNT from native strains of Mendoza soils open new perspectives for therapy against solid tumors.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Tech Science Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
BOTULINUM NEUROTOXIN  
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BREAST CANCER  
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CYTOSKELETON  
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Biología Celular, Microbiología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Effect of botulinum neurotoxins from Mendoza of clostridium botulinum strains on cytoskeletal proteins of mammary tumor cells  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
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info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-11-04T15:10:21Z  
dc.identifier.eissn
1667-5746  
dc.journal.pagination
16-16  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Chapana, Agostina Lucía. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Guarniolo, D.. Universidad Nacional de Cuyo. Facultad de Cs.médicas. Departamento de Patología. Area de Microbiología; Argentina  
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Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina  
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Fil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Cs.médicas. Departamento de Patología. Area de Microbiología; Argentina  
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Fil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Cs.médicas. Departamento de Patología. Area de Microbiología; Argentina  
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Fil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina  
dc.description.fil
Fil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Cs.médicas. Departamento de Patología. Area de Microbiología; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/uploads/attached/file/20210806/20210806053124_35488.pdf  
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Autor  
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dc.coverage
Nacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
IV Reunión Conjunta de Sociedades de Biología de la República Argentina  
dc.date.evento
2020-09-09  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Journal  
dc.description.institucionOrganizadora
Sociedad de Biología de Cuyo  
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Sociedad Argentina de Biología  
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Sociedad de Biología de Rosario  
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Sociedad Chilena de Reproducción y Desarrollo  
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Asociación de Biología de Tucumán  
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Sociedad de Biología de Córdoba  
dc.source.revista
Biocell  
dc.date.eventoHasta
2020-09-15  
dc.type
Reunión