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dc.contributor.author
Colonnella, Maria Antonela  
dc.contributor.author
Paris, Gastón  
dc.contributor.author
Lizarraga, Leonardo  
dc.date.available
2023-05-02T10:30:19Z  
dc.date.issued
2020  
dc.identifier.citation
Effect of nano-micrometric topographies on early steps of biofilm formation; XII Congreso Argentino de Microbiología General; Tucumán; Argentina; 2017; 1-1  
dc.identifier.uri
http://hdl.handle.net/11336/195891  
dc.description.abstract
Biofilms are defined as communities of microorganisms that live attached to a surface. They can include a single bacterial specie or multiple species and are formed on both abiotic and biotic surfaces. Thiswell-known phenomenon has undesirable effects for industrial or medical surfaces. Surface properties impact on the first steps of biofilm formation. Nature offers multiple solutions to biofilm formation. Animportant number of biological surfaces prevent microbial colonization due to their surface topographies, e.g.: the shells of mollusks and crabs and the skin of marine mammals and sharks. These facts have encouraged research of bioinspired surface designs. The main objectives of this work were to produce micro-nanometric hierarchical topographies and to analyze the influence of the topography on the bacterial adhesion. The hierarchical surface was designed using surface plasma oxidation of uni-axial stretch of polydimethylsiloxane (PDMS) films. This method has the advantage to allow designing sub-micrometric wrinkle topographic surfaces changing the plasma time exposition and the uniaxial stretch. Different topography surfaces were obtained, surface has wrinkles with different wavelength (from 500 to 3000 nm) and amplitude (from 80 to 700 nm) parameters. The bacterial adhesion on these novel hierarchical surfaces was evaluated through exposing them to a culture of Pseudomonas protegens Pf-5 for different times. The bacterial attachment was evaluated taking images of the wrinkled and smooth surfaces using an Atomic Force Microscopy (AFM). The initial results of this study suggests thatwrinkled surface with a wavelength of 1000 nm (aprox. bacteria size) delay the bacterial adhesion and, on the other hand, wrinkled surface with a wavelength of 3000 nm enhance and encourage bacterial adhesion. These results demonstrate the importance of the topographic surface to inhibit or stimulatethe biofilm development.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedad Argentina de Microbiología General  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Biofilm  
dc.subject
Pseudomonas protegens  
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Nanostructures  
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AFM  
dc.subject.classification
Biología Celular, Microbiología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Effect of nano-micrometric topographies on early steps of biofilm formation  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-11-09T19:41:33Z  
dc.journal.pagination
1-1  
dc.journal.pais
Argentina  
dc.journal.ciudad
Tucumán  
dc.description.fil
Fil: Colonnella, Maria Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina  
dc.description.fil
Fil: Paris, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina  
dc.description.fil
Fil: Lizarraga, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/108-Libro%20SAMIGE%202017.pdf  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Nacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
XII Congreso Argentino de Microbiología General  
dc.date.evento
2017-08-02  
dc.description.ciudadEvento
Tucumán  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General  
dc.source.libro
Libro de resúmenes: XII Congreso Argentino de Microbiología General  
dc.date.eventoHasta
2017-08-04  
dc.type
Congreso