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dc.contributor.author
Boron, Carlos Ignacio  
dc.contributor.author
Wirth, Sonia Alejandra  
dc.contributor.author
Battaglini, Fernando  
dc.date.available
2017-07-04T21:19:28Z  
dc.date.issued
2017-02  
dc.identifier.citation
Boron, Carlos Ignacio; Wirth, Sonia Alejandra; Battaglini, Fernando; Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins; Wiley VCH Verlag; Electroanalysis; 29; 2; 2-2017; 616-621  
dc.identifier.issn
1040-0397  
dc.identifier.uri
http://hdl.handle.net/11336/19553  
dc.description.abstract
Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Through adequate laccase immobilization, disposable screen printed electrodes can be used as interphase to build amperometric phenol sensors. In this work three different laccases were studied for the determination of phenol-like compounds, two of them are isoenzymes from Trametes trogii and the third one from Rhus vernicifera. Their immobilization on screen printed electrodes is presented for the construction of amperometric sensors. The electrode substrate is composed by graphite screen printed electrodes modified with carbon nanotubes and silica microspheres where, depending on the application, one of the three laccases is adsorbed. As each laccase shows an optimum working pH, they were conveniently selected to determine dopamine at physiological pH and catechol at acid pH. Determinations in the micromolar range were possible in both cases. Chronoamperometry shows to be an effective technique for their determinations, simpler than other electrochemical methods already presented in the literature.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley VCH Verlag  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Phenol Derivatives  
dc.subject
Laccase  
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Screen Printed Electrode  
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Dopamine  
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Ph Dependence  
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-07-04T15:34:26Z  
dc.journal.volume
29  
dc.journal.number
2  
dc.journal.pagination
616-621  
dc.journal.pais
Alemania  
dc.description.fil
Fil: Boron, Carlos Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina  
dc.description.fil
Fil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina  
dc.description.fil
Fil: Battaglini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina  
dc.journal.title
Electroanalysis  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/elan.201600470  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.201600470/abstract