Artículo
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
Lasagno, Mirta Cristina; Reinoso, Elina Beatríz
; Dieser, Silvana Andrea
; Calvinho, Luis Fernando; Buzzola, Fernanda Roxana
; Bogni, Cristina Ines; Odierno, Liliana Mónica
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
07/2011
Editorial:
Asociación Argentina de Microbiología
Revista:
Revista Argentina de Microbiología
ISSN:
0325-7541
e-ISSN:
1851-7617
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberiswere isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. TheS. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains
expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profileswere identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similaritywere identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberisstrains with the same PFGE pattern showed different virulence profiles. Bovine S. uberisstrains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypiccharacteristics, and the PFGE patternsare
not associated with the virulence profiles.
Palabras clave:
STREP. UBERIS
,
BOVINA SUBCLINICAL MASTITIS
,
PFGE
,
VIRULENCE FACTORS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Lasagno, Mirta Cristina; Reinoso, Elina Beatríz; Dieser, Silvana Andrea; Calvinho, Luis Fernando; Buzzola, Fernanda Roxana; et al.; Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 43; 3; 7-2011; 212-217
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