Artículo
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
Fecha de publicación:
04/2012
Editorial:
American Association of Avian Pathologists
Revista:
Avian Diseases
ISSN:
0005-2086
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcriptionpolymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services LaboratoryU.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F 117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
Palabras clave:
GENETIC CHARACTERIZATION
,
PHYLOGENETIC ANALYSIS
,
PIGEON PARAMYXOVIRUS
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Castro, E. R.; Zanetti, Flavia Adriana; Arbiza, Juan; Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay; American Association of Avian Pathologists; Avian Diseases; 56; 1; 4-2012; 243-248
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