Mostrar el registro sencillo del ítem
dc.contributor.author
Abdulhamid, María Belén
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Baigori, Mario Domingo
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Pera, Licia Maria
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2023-04-24T13:31:19Z
dc.date.issued
2015
dc.identifier.citation
Impact of manganese on the production of a biomass associated β-D-glucosidase activity using a thermophilic Bacillus licheniformis strain; XI Congreso Argentino de Microbiología General; Cordoba; Argentina; 2015; 69-69
dc.identifier.uri
http://hdl.handle.net/11336/195121
dc.description.abstract
The b-Glucosidase (EC 3.2.1.21) constitutes a group of well-studied hydrolases. This enzyme catalyzes the hydrolysis of arylglucosides, alkylglucosides, cellobiose, and cellooligosaccharides. The interest in this biocatalyst centers on its roles in the enzymatic hydrolysis of cellulose. The rate of cellulose hydrolysis can be improved by supplementing commercial cellulases with immobilized b-D-glucosidase, which usually has high stability and can be recovered and reused. In addition, for industrial saccharification of cellulosic materials, b-glucosidases from thermophilic bacteria are also of particular interest due to their increased stability. In this work, we study the influence of manganese on the production of a biomass associate b-D-glucosidase activity using a thermophilic Bacillus licheniformis strain. Assays were performed at 45 °C in 500 ml Erlenmeyer containing 200 ml of LB medium supplemented with 0 - 1.0 mM MnCl2 . The b-D-glucosidase activity was also determined at 45 °C using 3.6 mM p-nitrophenyl-b -D-glucopyranoside (Sigma) as substrate. Cells were harvested by centrifugation and washed twice with 100 mM Tris-HCl buffer (pH 7). The pellet was resuspended in the same buffer, and it was directly used as the b-D-glucosidase source. The mixture was shaken at 1000 rpm. Then, the absorbance of the supernatant was measured at 405 nm and the enzyme activity calculated and related to the biomass dry weight. One unit of the enzyme was defined as the amount needed to release 1 µmol p-nitrophenol per min. Thus, dose-response experiments showed that in the presence of 0.3 mM MnCl2 the enzyme production was increased by about 20%. Under this culture condition, a specific activity value of 19.99 U per mg of dry weight was obtaining after 4 h of cultivation. Finally, these results could be of relevance to the bioethanol industry where lignocellulosic material is used as feedstock for fermentation and, which should be treated enzymatically. The use of naturally bound enzymes is an important immobilization technique. This type of biocatalyst system is potentially cost-effective because the biomass can be directly utilized in the treatment.
dc.format
application/pdf
dc.language.iso
spa
dc.publisher
Sociedad Argentina de Microbiología General
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Production
dc.subject
β-D-glucosidase associated to the biomass
dc.subject
Bacillus
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Biotecnología Industrial
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Impact of manganese on the production of a biomass associated β-D-glucosidase activity using a thermophilic Bacillus licheniformis strain
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-27T15:58:37Z
dc.journal.pagination
69-69
dc.journal.pais
Argentina
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.description.fil
Fil: Abdulhamid, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2015.pdf
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
XI Congreso Argentino de Microbiología General
dc.date.evento
2015-08-05
dc.description.ciudadEvento
Cordoba
dc.description.paisEvento
Argentina
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General
dc.source.libro
Libro de Resumenes de XI Congreso Argentino de Microbiología General
dc.date.eventoHasta
2015-08-07
dc.type
Congreso
Archivos asociados