Evento
The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
Pescaretti, María de Las Mercedes
; Lopez, Fabian Enrique
; Morero, Roberto Dionisio
; Delgado, Monica Alejandra
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Tipo del evento:
Reunión
Nombre del evento:
XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Fecha del evento:
10/11/2009
Institución Organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular;
Título de la revista:
Biocell
Editorial:
Tech Science Press
ISSN:
0327-9545
e-ISSN:
1667-5746
Idioma:
Español
Clasificación temática:
Resumen
The Rcs phosphorelay is a adaptive response system composed of three proteins: the sensor RcsC, the cognate response regulator RcsB; and the intermediary in the phosphoryl transfer RcsD. Has been described that the flow of phosphoryl groups through the Rcs phosphorelay can occur in following way RcsC/RcsD/RcsB. At present, the signal that activates the Rcs system has not been determined. We have demonstrated in a previous study that the overexpression of rcsB modulates the RcsB-dependent genes in an rcsC or rcsD mutant but not in the doble mutant. These results suggest that only RcsB-P, the RcsB phosphorylated form, is able to induce this modulation, and we postulated that the RcsB-P would occur by two or more different pathways. The goal of present work was study the possible phosphorelay pathways. In this work we used the two-hybrid assay (BACTH), consisting of the reconstitution of the adenylate cyclase activity in E. coli when two target proteins interacts in vivo in bacteria, measured by b-galactosidase activity. In this assay we probe the RcsC, RcsD and RcsB interaction to define the Rcs phosphorelay transduction mechanism.
Palabras clave:
RCSCDB
,
SYSTEM
,
PROTEIN
,
INTERACTION
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(PROIMI)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 100-100
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