Artículo
Changes in membrane lipid composition cause alterations in epithelial cell-cell adhesion structures in renal papillary collecting duct cells
Marquez, Maria Gabriela
; Favale, Nicolas Octavio
; Leocata Nieto, Francisco; Pescio, Lucila Gisele
; Sterin, Norma Beatriz
Fecha de publicación:
03/2012
Editorial:
Elsevier Science
Revista:
Biochimica et Biophysica Acta - Biomembranes
ISSN:
0005-2736
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In epithelial tissues, adherens junctions (AJ) mediate cell-cell adhesion by using proteins called E-cadherins, which span the plasma membrane, contact E-cadherin on other cells and connect with the actin cytoskeleton inside the cell. Although AJ protein complexes are inserted in detergent-resistant membrane microdomains, the influence of membrane lipid composition in the preservation of AJ structures has not been extensively addressed. In the present work, we studied the contribution of membrane lipids to the preservation of renal epithelial cell-cell adhesion structures. We biochemically characterized the lipid composition of membranes containing AJ complexes. By using lipid membrane-affecting agents, we found that such agents induced the formation of new AJ protein-containing domains of different lipid composition. By using both biochemical approaches and fluorescence microscopy we demonstrated that the membrane phospholipid composition plays an essential role in the in vivo maintenance of AJ structures involved in cell-cell adhesion structures in renal papillary collecting duct cells.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Marquez, Maria Gabriela; Favale, Nicolas Octavio; Leocata Nieto, Francisco; Pescio, Lucila Gisele; Sterin, Norma Beatriz; Changes in membrane lipid composition cause alterations in epithelial cell-cell adhesion structures in renal papillary collecting duct cells; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1818; 3; 3-2012; 491-501
Compartir
Altmétricas