Artículo
Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
Dudiuk, Catiana Beatriz
; Gamarra, Soledad; Jimenez Ortigoza, Cristina; Leonardelli, Florencia
; Macedo, Daiana; Perlin, David S.; Garcia, Guillermo Manuel
Fecha de publicación:
07/2015
Editorial:
American Society for Microbiology
Revista:
Journal of Clinical Microbiology
ISSN:
0095-1137
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.
Palabras clave:
Candida
,
Albicans
,
Echinocandins
,
Resistance
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Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Dudiuk, Catiana Beatriz; Gamarra, Soledad; Jimenez Ortigoza, Cristina; Leonardelli, Florencia; Macedo, Daiana; et al.; Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans; American Society for Microbiology; Journal of Clinical Microbiology; 53; 7; 7-2015; 2037-2041
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