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dc.contributor.author
Sosa Alderete, Lucas Gastón
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dc.contributor.author
Agostini, Elizabeth
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dc.contributor.author
Medina, Maria Inés
dc.date.available
2023-04-12T15:15:28Z
dc.date.issued
2011-09
dc.identifier.citation
Sosa Alderete, Lucas Gastón; Agostini, Elizabeth; Medina, Maria Inés; Antioxidant response of tobacco (Nicotiana tabacum) hairy roots after phenol treatment; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 49; 9; 9-2011; 1020-1028
dc.identifier.issn
0981-9428
dc.identifier.uri
http://hdl.handle.net/11336/193496
dc.description.abstract
Phenol is released to the environment from a wide variety of industrial effluents and it causes severe problems to human health and ecosystem. In the present study, we determined that Nicotiana tabacum hairy roots (HRs) double transgenic (DT) for two peroxidase genes (tpx1 and tpx2) showed higher phenol removal efficiency than wild type (WT) HRs after 120 h of phenol treatment at the expense of endogenous H 2O 2. Besides, to determine whether phenol could induce oxidative stress on tobacco HRs, we analyzed the antioxidant response, superoxide anion (O 2 -) localization and malondialdehyde (MDA) levels. Both HRs treated with phenol, showed significant increases in peroxidase (PX) activity mainly at the end of the assay (120 h) being PX activity from transgenic HRs 40% higher than that of WT HRs. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities showed significant increases from 24 to 120 h of phenol treatment. PX, SOD and APX isoforms were also analyzed and slight changes were observed only in PX patterns. Both HRs showed significant differences in total glutathione (TGSH) content during treatment, being higher in DT HRs than in WT HRs. At the end of the assay, a greater accumulation of O 2 - in different root zones was observed in WT and DT HRs. Moreover, phenol was able to increase the MDA levels in WT HRs from 48 to 120 h of the treatment, but no significant changes were observed in DT HRs. Results suggest that under these experimental conditions, DT HRs would be more tolerant to phenol than WT HRs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier France-Editions Scientifiques Medicales Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
OXIDATIVE STRESS
dc.subject
PHENOL
dc.subject
TRANSGENIC HAIRY ROOTS
dc.subject.classification
Biotecnología Medioambiental
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dc.subject.classification
Biotecnología del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Antioxidant response of tobacco (Nicotiana tabacum) hairy roots after phenol treatment
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-04-11T14:33:58Z
dc.journal.volume
49
dc.journal.number
9
dc.journal.pagination
1020-1028
dc.journal.pais
Francia
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dc.journal.ciudad
Paris
dc.description.fil
Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
dc.description.fil
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
dc.description.fil
Fil: Medina, Maria Inés. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
dc.journal.title
Plant Physiology and Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2011.07.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0981942811002154
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