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Scalise, Maria Lujan
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Garimano, Nicolás Ezequiel
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Porporato, Melina Mabel
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Leonino, Patricia
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Pereyra, Adriana
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Ferreiros, José A.
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Casale, Roberto
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Amaral, María Marta
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Ibarra, Cristina Adriana
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Sacerdoti, Flavia
dc.date.available
2023-04-10T15:32:53Z
dc.date.issued
2021
dc.identifier.citation
Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; International Symposium on Reproductive Health; Virtual Meeting; Singapur; 2021; 742-743
dc.identifier.issn
2709-0094
dc.identifier.uri
http://hdl.handle.net/11336/193057
dc.description.abstract
Brief Introduction: Some studies have demonstrated that vaginal E. coli colonization may produce complicationsduring pregnancy. We have previously reported that Shiga toxin-2 (Stx2) secreted by enterohemorrhagic E. coli canproduce abortion and premature delivery in animals and can impair human trophoblast in vitro.Objective: The aim of this study was to detect virulence factor genes from Shiga toxin producing E. coli (STEC)in the endocervix of asymptomatic pregnant women. Methods: Endocervical swabs were collected from 103 pregnantwomen (12 to 30 weeks of pregnancy) during their antenatal examination. Swab samples were enriched in TrypticSoy Broth overnight at 37 °C and then streaked into selective and differential medium, Sorbitol MacConkey agar. E.coli detection was confirmed by identification of uidA gene by PCR assay. Then, positive samples for E. coli wereanalyzed for STEC virulence factors genes: stx1, stx2, eae, rfbO157, lpfAO113 and hcpA. After that, positive E. colisamples for stx2 gene were grown in Luria-Bertani Broth medium in order to evaluate Stx2 cytotoxic activity. Forthat, the bacterial supernatants were filter-sterilized and cytotoxicity was evaluated on Vero, Swan 71 and HeLa cells.Results: Our results showed that 14.5% of the endocervical samples were positive for E. coli (positive for uidAgene). Furthermore, 9/15 (60%) of the E. coli-positive samples carried the stx2 gene and 6/15 (40%) carried thelpfAO113 and hcpA genes. One bacterial supernatant from an E. coli-positive endocervical sample carrying stx2 andlpfAO113 genes exhibited high cytotoxic activity on the cells evaluated due to Stx2 production confirmed by specificneutralization of Stx2 with an anti-Stx2 antibody.Conclusions: The novelty of this report is the presence of STEC in the endocervix of asymptomatic pregnantwomen. This opens a new perspective with respect to the possible role of this pathogen in woman reproductivehealth care.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
IMR Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HUS
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Pregnancy
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Stx2
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
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info:eu-repo/semantics/publishedVersion
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info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-11-09T18:52:46Z
dc.journal.volume
48
dc.journal.number
3
dc.journal.pagination
742-743
dc.journal.pais
Singapur
dc.journal.ciudad
Singapur
dc.description.fil
Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
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Fil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
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Fil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
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Fil: Leonino, Patricia. Hospital Nacional Profesor Alejandro Posadas.; Argentina
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Fil: Pereyra, Adriana. Hospital Nacional Profesor Alejandro Posadas.; Argentina
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Fil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas.; Argentina
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Fil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas.; Argentina
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Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.description.fil
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.description.fil
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://imrpress.com/journal/CEOG/48/3/10.31083/j.ceog.2021.03.0511
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dc.coverage
Internacional
dc.type.subtype
Simposio
dc.description.nombreEvento
International Symposium on Reproductive Health
dc.date.evento
2021-05-07
dc.description.ciudadEvento
Virtual Meeting
dc.description.paisEvento
Singapur
dc.type.publicacion
Journal
dc.description.institucionOrganizadora
The Physicologycal Society
dc.description.institucionOrganizadora
Biochemical Society
dc.source.revista
Clinical and Experimental Obstetrics & Gynecology
dc.date.eventoHasta
2021-05-07
dc.type
Simposio
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