Artículo
Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
Pilili, Juan Pablo
; González, Norma Viviana; Molinari, Gabriela Beatriz
; Reigosa, Miguel Antonio
; Soloneski, Sonia Maria Elsa
; Larramendy, Marcelo Luis
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
01/2010
Editorial:
Academic Press Ltd - Elsevier Science Ltd
Revista:
Biologicals
ISSN:
1045-1056
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, χ2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, χ2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Pilili, Juan Pablo; González, Norma Viviana; Molinari, Gabriela Beatriz; Reigosa, Miguel Antonio; Soloneski, Sonia Maria Elsa; et al.; Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera; Academic Press Ltd - Elsevier Science Ltd; Biologicals; 38; 1; 1-2010; 135-143
Compartir
Altmétricas