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dc.contributor.author
Torreggiani, A.
dc.contributor.author
Barata Vallejo, Sebastian
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dc.contributor.author
Chatgilialoglu, C.
dc.date.available
2023-04-05T17:57:18Z
dc.date.issued
2011-07
dc.identifier.citation
Torreggiani, A.; Barata Vallejo, Sebastian; Chatgilialoglu, C.; Combined Raman and IR spectroscopic study on the radical-based modifications of methionine; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 401; 4; 7-2011; 1231-1239
dc.identifier.issn
1618-2642
dc.identifier.uri
http://hdl.handle.net/11336/192912
dc.description.abstract
Among damages reported to occur on proteins, radical-based changes of methionine (Met) residues are one of the most important convalent post-translational modifications. The combined application of Raman and infrared (IR) spectroscopies for the characterisation of the radical-induced modifications of Met is described here. Gamma-irradiation was used to simulate the endogenous formation of reactive species such as hydrogen atoms ( •H), hydroxyl radicals ( •OH) and hydrogen peroxide (H 2O 2). These spectroscopic techniques coupled to mass experiments are suitable tools in detecting almost all the main radical-induced degradation products of Met that depend on the nature of the reactive species. In particular, Raman spectroscopy is useful in revealing the radical-induced modifications in the sulphur-containing moiety, whereas the IR spectra allow decarboxylation and deamination processes to be detected, as well as the formation of other degradation products. Thus, some band patterns useful for building a library of spectra-structure correlation for radical-based degradation of Met were identified. In particular, the bands due to the formation of methionine sulfoxide, the main oxidation product of Met, have been identified. All together, these results combine to produce a set of spectroscopic markers of the main processes occurring as a consequence of radical stress exposure, which can be used in a spectroscopic protocol for providing a first assessment of Met modifications in more complex systems such as peptides and proteins, and monitoring their impact on protein structure.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Heidelberg
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GAMMA-RADIOLYSIS
dc.subject
IR SPECTROSCOPY
dc.subject
METHIONINE
dc.subject
RADICAL STRESS
dc.subject
RAMAN SPECTROSCOPY
dc.subject.classification
Química Orgánica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Combined Raman and IR spectroscopic study on the radical-based modifications of methionine
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-04-05T15:39:11Z
dc.journal.volume
401
dc.journal.number
4
dc.journal.pagination
1231-1239
dc.journal.pais
Alemania
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dc.journal.ciudad
Stuttgart
dc.description.fil
Fil: Torreggiani, A.. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Barata Vallejo, Sebastian. Consiglio Nazionale delle Ricerche; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Chatgilialoglu, C.. Consiglio Nazionale delle Ricerche; Italia
dc.journal.title
Analytical and Bioanalytical Chemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00216-011-5203-0
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00216-011-5203-0
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