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dc.contributor.author
Suarez, Carlos E.
dc.contributor.author
Laughery, Jacob M.
dc.contributor.author
Bastos, Reginaldo G.
dc.contributor.author
Johnson, Wendell C.
dc.contributor.author
Norimine, Junzo
dc.contributor.author
Asenzo, Gustavo Daniel
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dc.contributor.author
Brown, Wendy C.
dc.contributor.author
Jacobsen, Monica Ofelia
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dc.contributor.author
Goff, Will L.
dc.date.available
2023-04-05T12:37:36Z
dc.date.issued
2011-06
dc.identifier.citation
Suarez, Carlos E.; Laughery, Jacob M.; Bastos, Reginaldo G.; Johnson, Wendell C.; Norimine, Junzo; et al.; A novel neutralization sensitive and subdominant RAP-1-related antigen (RRA) is expressed by Babesia bovis merozoites; Cambridge University Press; Parasitology; 138; 7; 6-2011; 809-818
dc.identifier.issn
0031-1820
dc.identifier.uri
http://hdl.handle.net/11336/192806
dc.description.abstract
Objective. The Babesia bovis genome encodes a rap-1 related gene denominated RAP-1 related antigen (RRA). In this study, we analysed the pattern of expression, immunogenicity and functional relevance of RRA. Methods. Phylogenetic analysis was performed using the program Phylip. Expression of rra was analysed by Northern blots, RT-PCR, immunoprecipitation, Western blots and immunofluorescence. RRA antigenicity was tested by T-cell proliferation and Western blot analysis, and functional relevance was determined in an in vitro neutralization assay. Results. RRA is more closely related to RAP-1b of Babesia bigemina than to B. bovis RAP-1, and it is highly conserved among distinct strains. Transcriptional analysis suggests lower numbers of rra transcripts compared to rap-1. Immunoprecipitation of metabolically labelled B. bovis proteins with antibodies against synthetic peptides representing predicted antigenic regions of RRA confirmed the expression of a ∼43 kDa RRA in cultured merozoites. Antibodies present in B. bovis hyperimmune sera, but not in field-infected cattle sera, reacted weakly with recombinant RRA, and no significant stimulation was obtained using recombinant RRA as antigen in T-cell proliferation assays, indicating that RRA is a subdominant antigen. Antibodies against RRA synthetic peptides reacted with merozoites using immunofluorescence, and were able to significantly inhibit erythrocyte invasion in in vitro neutralization tests, suggesting functional relevance for parasite survival. Conclusion. B. bovis express a novel subdominant RAP-1-like molecule that may contribute to erythrocyte invasion and/or egression by the parasite.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
APICOMPLEXAN
dc.subject
BABESIA BOVIS
dc.subject
RAP-1
dc.subject
RHOPTRY-ASSOCIATED PROTEIN-1
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
A novel neutralization sensitive and subdominant RAP-1-related antigen (RRA) is expressed by Babesia bovis merozoites
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-04-03T12:10:32Z
dc.journal.volume
138
dc.journal.number
7
dc.journal.pagination
809-818
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Suarez, Carlos E.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos. Washington State University; Estados Unidos
dc.description.fil
Fil: Laughery, Jacob M.. Washington State University; Estados Unidos
dc.description.fil
Fil: Bastos, Reginaldo G.. Washington State University; Estados Unidos
dc.description.fil
Fil: Johnson, Wendell C.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
dc.description.fil
Fil: Norimine, Junzo. Washington State University; Estados Unidos
dc.description.fil
Fil: Asenzo, Gustavo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
dc.description.fil
Fil: Brown, Wendy C.. Washington State University; Estados Unidos
dc.description.fil
Fil: Jacobsen, Monica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
dc.description.fil
Fil: Goff, Will L.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
dc.journal.title
Parasitology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/abs/novel-neutralization-sensitive-and-subdominant-rap1related-antigen-rra-is-expressed-by-babesia-bovis-merozoites/23E4805051C7492627F02CAB4F3EB8A0
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1017/S0031182011000321
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